血管紧张素II对人肾成纤维细胞增殖的影响

王俭勤; 韦洮; 于仲元

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中国血液净化 ›› 2004, Vol. 3 ›› Issue (2) : 94-96.

基础研究

血管紧张素II对人肾成纤维细胞增殖的影响

王俭勤韦洮于仲元

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摘要

目的观察血管紧张素(AgII)对培养的人肾间质成纤维细胞(HRIF )增殖的影响。方法分离培养的HRIF经鉴定后，利用3H -TDR掺入法，流式细胞仪及免疫组织化学技术观察了AgII 对HRIF DNA合成、细胞周期的影响。结果 ①AgII 10-10M至10-6M均增加了HRIF3 H-TDR掺入率，第1、3天呈剂量依赖关系(γ1=0.709， P<0.010； γ3=0.806， P<0.010)。② AgII(10-8M至10-5M)明显促进了HRIFG1期向S期转化( P<0.01)。结论 AgII能够直接诱导HRIF细胞的增殖。

Abstract

Objective Observing the influence of angiotensin II on human renal interstitial fibroblast(HRIF) proliferation. Investigating the role of angiotensin II in the progress of kidney prolifrative diseases at the cell level. Methods Separated and cultured renal interstitial fibroblast were identified. To observe the effect of angiotensin II on the cell cycle and DNA synthesis of HRIF and the inhibitive effect of Losartan, the 3 H-TDR incorporation, flowcytometry and immunohistochemical technique were applied. Result ① Angiotensin II (10-10M～10-6M) has elevated the incorporation rate of HRIF 3H-TDR and has showed dose-dependence in day 1 and day 3 (γ1=0.709 P<0.010；γ3=0.806，P<0.010). ②Angiotensin II (10-8M～10-5M) has markly promoted the transformaion of HRIF from G1 stage to S stage (P<0.010). Conclusion. AgII can induce the proliferation of HRIF directly.