关键词: 培养, 胆红素, 生物材料

Abstract: Objective We probed the basic culture methods of adult animal hepatocytes(AAH) which may become more ideal biological material of extracorporeal bioartificial liver support system (EBLSS) and their biological activity of metabolism of bilirubin and synthesis of carbamide. Methods ①Using three kinds of basic components (synthetic medium, serum and insulin), the 15 kinds of combination of cell-cultured methods on adult mice hepatocytes were observed. ② AAH were cultured with hyperbilirubin serum of human severe hepatitis and their biological activities of metabolism of bilirubin and synthesis of carbamide were observed. Results Cells cultured in high metabolism density culture medium and containing 20% inactivated allograph male rat serum，10U/ml insulin, high sugar DMEM still remained 20% survival rate in the 14th day. The metabolic activity of 24 hours-pre-cultured monolayer hepatocytes was the most powerest on bilirubin. The Serum total bilirubin, direct bilirubin and indirect bilirubin fell 28.88%, 21.13%, 33.63% respectively after 3 hours culturing by hyperbilirubin serum of human severe hepatitis. Conclusion This model system can be used as the basic culture medium of adult animal hepatocytes. AAH would become more ideal biological material of EBLSS to substitute the newly born animal hepatocytes and embryo hepatocytes.

Key words: Bilirubin, Biological material