关键词: 肾囊内注射, 基因导入, 基因表达, 基因治疗, β-半乳糖苷酶基因

Abstract:

Objective Renal adipose capsule is an adipose tissue around the kidney. It has certain volume and can be acted as a storing place of some medications and then release them into renal tissue. We injected the gene into the renal adipose capsule, then observed whether the gene could be expressed in the kidneys locally and specifically, in order to evaluate a new gene therapeutic technique to treat some renal diseases through local gene expression.
Methods We chose 15 healthy male SD rats, using β-Galactosidase gene, combined the gene with the vector, liposome, then injected the DNA-liposome complex into the renal adipose capsule. At 12 hours, 24 hours, 36 hours, 48 hours, 72 hours and 7 days after injection, we observed the gene expression in the kidneys, and in other organs such as heart, liver, spleen, lung, muscle, and so on. Results At 12 hours after injecting β-Galactosidase gene into the renal adipose capsule, the galactosidase expression could be seen in the kidneys, and the expression lasted for 7 days, but no expression was found in the other organs. The expression was located in the renal tubules, no expression was found in the glomerulus. Conclusions Our findings support that the gene could be expressed in the kidneys after intra-renal adipose capsule injection of plasmid DNA. Local gene expression after gene injection into renal adipose capsule may be an effective means of delivering therapeutic gene to the kidney. It will open a new way for gene treatment of some renal diseases.

Key words: Gene transfer, Gene expression, Gene therapy, β-Galactosidase gene 