中国血液净化

›› 2005, Vol. 4 ›› Issue (7): 379-380.

• 基础研究 • 上一篇    下一篇

高糖、尿毒症血清对人腹膜间皮细胞VEGF表达的影响

于 青 赵东杰 徐 琦 姚 建   

  1. 200080 上海，上海交通大学附属第一人民医院肾内科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-07-12 发布日期:2005-07-12

  • Received:1900-01-01 Revised:1900-01-01 Online:2005-07-12 Published:2005-07-12

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摘要： 目的 高糖、尿毒症血清对人腹膜间皮细胞VEGF表达的影响。方法 用逆转录聚合酶链反应（RT-PCR）和免疫组化方法来观察高糖、尿毒症血清对人腹膜间皮细胞VEGF表达的影响。结果 高糖、尿毒症血清组VEGF表达明显高于正常对照组。结论 高糖、尿毒症血清可促进人腹膜间皮细胞VEGF的表达，可能导致腹膜新生血管的形成，从而引起腹膜超滤衰竭。

关键词: 人腹膜间皮细胞, 血管内皮生长因子, 高糖, 尿毒症血清

Abstract: Objective To study the effects of high glucose and uremic serum on the expression of VEGF in human peritoneal mesothelial cell. Methods Reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry were applied to detect the expression of VEGF. Results The increased expression of peritoneal VEGF was found in high glucose and uremic serum groups compared with normal group. Conclusions High glucose or uremic serum upregulates the expression of peritoneal VEGF and may induce the increasement of neoangiogensis, this may cause the incidence of peritoneal ultrafiltration failure.

Key words: Vascular endothelial growth factor, High glucose, Uremic serum

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