高糖、尿毒症血清对人腹膜间皮细胞VEGF表达的影响

于青; 赵东杰; 徐琦; 姚建

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中国血液净化 ›› 2005, Vol. 4 ›› Issue (7) : 379-380.

基础研究

高糖、尿毒症血清对人腹膜间皮细胞VEGF表达的影响

于青赵东杰徐琦姚建

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摘要

目的高糖、尿毒症血清对人腹膜间皮细胞VEGF表达的影响。方法用逆转录聚合酶链反应（RT-PCR）和免疫组化方法来观察高糖、尿毒症血清对人腹膜间皮细胞VEGF表达的影响。结果高糖、尿毒症血清组VEGF表达明显高于正常对照组。结论高糖、尿毒症血清可促进人腹膜间皮细胞VEGF的表达，可能导致腹膜新生血管的形成，从而引起腹膜超滤衰竭。

Abstract

Objective To study the effects of high glucose and uremic serum on the expression of VEGF in human peritoneal mesothelial cell. Methods Reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry were applied to detect the expression of VEGF. Results The increased expression of peritoneal VEGF was found in high glucose and uremic serum groups compared with normal group. Conclusions High glucose or uremic serum upregulates the expression of peritoneal VEGF and may induce the increasement of neoangiogensis, this may cause the incidence of peritoneal ultrafiltration failure.