全反式维甲酸对单侧输尿管梗阻大鼠肾脏的作用

朱丹; 桂保松; 卫小红; 姚钢炼; 高登峰; 宁宁; 张红莉

PDF(232 KB)

中国血液净化 ›› 2005, Vol. 4 ›› Issue (7) : 381-383.

基础研究

全反式维甲酸对单侧输尿管梗阻大鼠肾脏的作用

朱丹桂保松卫小红姚钢炼高登峰宁宁张红莉

作者信息 +

文章历史 +

摘要

目的　研究全反式维甲酸(all-trans-retinoic acid，at-RA)在单侧输尿管梗阻(unilateral uretreal obstructive，UUO) 大鼠模型中对肾间质肌成纤维细胞(MyoF) 的积聚及胶原Ⅲ(col Ⅲ) 沉积的影响。方法　将40只大鼠随机分为假手术组、UUO组、维甲酸大剂量组，维甲酸小剂量组，苯那普利组。后3组分别给予全反式维甲酸20mg、10mg·kg-1·d-1和苯那普利10mg·kg-1·d-1。术后第14天处死各组大鼠,用免疫组化方法测定α平滑肌肌动蛋白(αSMA) 及colⅢ的表达。行HE、MASSON染色观察肾脏病理变化。结果　全反式维甲酸可显著减少肾间质区MyoF积聚，减轻colⅢ的沉积,并改善肾脏的病理学变化。结论　全反式维甲酸可通过减少肾间质区MyoF积聚,减轻col Ⅲ的沉积而改善UUO 所致的肾间质纤维化。

Abstract

Objective To study the effect of all-transretinoic acid(at-RA) on the process of renal interstitial fibrosis following unilateral ureteral obstruction (UUO) in rats. Methods Fouty SD rats were randomly assigned into 5 groups: Two atRA treatment groups (10mg and 20mg·kg-1·d-1 respectively), benazepril treatment group(10mg·kg-1·d-1), UUO control group and normal control group. All of rats were killed 14 days after surgery. Immunohistochemistry was performed on renal tissue to detect smooth muscle actin(α-SMA) which was a cytoskeletal marker of myofibroblast (MyoF) and collagen Ⅲ(col Ⅲ). Histological studies were also done by he and MASSON stainting. Results　Fourteen days after surgery, interstitial myof infiltration and interstitial colⅢdeposition were all significantly reduced by at-RA treatment. At-RA treatment also improved the histological changes of UUO rats. Conclusion Reduction of interstitial MyoF infiltration may be an important event by which at-RA prevents renal injury caused by UUO.