TGF-β1诱导人肾小管上皮细胞血小板反应蛋白1的表达

邹贵勉; 张璟; 周飞舟

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中国血液净化 ›› 2005, Vol. 4 ›› Issue (7) : 384-386.

基础研究

TGF-β1诱导人肾小管上皮细胞血小板反应蛋白1的表达

邹贵勉　张璟　周飞舟

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摘要

目的　探讨在TGF-β1诱导下人肾小管上皮细胞(HKCs)血小板反应蛋白1(thrombospondin 1, TSP-1）的表达变化。方法　实验分二组：对照组(C组)用无血清培养基(FSM)培养人肾小管上皮细胞；TGF-β1组(T组)：用含TGF-β1 20ng/ml的FSM培养HKCs。各组观察24、72和120h 3个时相点，分别为C1、C2、C3和T1、T2、T3亚组。用免疫细胞化学及逆转录酶链反应（RT-PCR）方法检测TSP-1 及其mRNA的表达的变化。结果　T1组比C1组TSP-1表达无显著差异( P＞0.05)，T2、T3组与C组相应时相点比较TSP-1表达显著增高(P＜0.01)，T组组内比较随时间延长，TSP-1表达逐渐增高。结论TGF-β1能诱导HKCs表达TSP-1，呈时间依赖性。

Abstract

Objective To investigate the influence of TGF-β1 on the expression of thrombospondin 1 (TSP-1) in human tubular epithelial cells. Methods Human tubular epithelial cell (HKCs) line were cultured in vitro with DMEM/F12 (1:1). HKCs were divided into tow groups: ①Normal control groups (C groups)：HKCs were cultured in free serum medium (FSM); ②TGF-β1 groups (Tgroups) : HKCs werecultured in FSM contained TGF-β1. All cells were redivided into there subgroups C1, C2, C3 and T1, T2, T3 by culturing 24, 72 and 120 hours respectively. The expression of TSP-1 were detected with Immuno- histochemistry. The expression of TSP-1 mRNA were analyzed by RT-PCR. The corelation between the relative culture time and TSP-1 mRNA were analyzed. Results TSP-1 expression were negative in C groups and T1 group. However, the expression were positive in T2 group and strong positive in T3 group compared with C2 and C3 groups respectively, P＜0.01. Statistical analysis showed that there was a positive correlation between culture time and TSP-1 mRNA expression (R=0.758, P＜0.01). Conclusions TGF-β1 could time dependenly induce the expression of TSP-1 in HKCs with.