中国血液净化

›› 2006, Vol. 5 ›› Issue (12): 822-825.

• 论著 • 上一篇    下一篇

腹膜透析感染期过氧化物酶体增殖物激活受体γ水平的变化及意义

解汝娟 隋满姝 贾西贝   

  1. 150001 哈尔滨，哈尔滨医科大学附属第一医院肾内科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-12-12 发布日期:2006-12-12

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-12-12 Published:2006-12-12

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摘要： 目的 探讨腹膜透析感染期过氧化物酶体增殖物激活受体γ水平的变化及意义。方法 选择哈尔滨医科大学附属第一医院肾内科腹膜透析患者，分为实验组与对照组，应用RT-PCR对实验组和对照组腹膜透析液及外周抗凝血提取的巨噬细胞PPARγmRNA表达水平进行测定,ELISA测定外周血单核细胞TNF-α、IL-6水平。结果 RT-PCR显示腹膜透析液及外周血单核细胞PPARγmRNA的表达水平，实验组第1天[分别为(0.52±0.06)，(0.68±0.07)]均明显低于实验组第5天[分别为(0.66±0.09)，(0.89±0.08)及对照组[分别为(0.68±0.08)，(0.93±0.06)](P＜0.01)。腹膜炎症初期,PPARγmRNA为低表达。ELISA测定外周抗凝血单核细胞TNF-α、IL-6水平，实验组第1天[分别为(20.16±11.26)ng/L，(26.89±13.46)ng/L]均明显高于实验组第5天[分别为(6.38±4.28)ng/L，(8.96±4.67)ng/L]及对照组[分别为(5.39±3.50)ng/L，(7.83±4.26)ng/L](P＜0.01)，与PPARγmRNA表达水平呈明显负相关。结论 腹膜透析合并腹膜炎初期患者腹腔巨噬细胞PPARγmRNA低表达,PPARγ在腹腔局部免疫中可能有重要意义。

关键词: 腹膜透析, 腹膜炎, PPARγ

Abstract: Objective To investigate the effect of dialysis-induced peritonitis on peroxisome proliferator-activated receptor(PPAR)-γ mRNA expression of peritoneal monocyte in peritoneal dialysis patients. Methods Thirty-nine peritoneal dialysis patients were divided into experimental group(EG，n=19) and control group (CG，n=20). The PPAR-γ gene expression of peripheral blood(PB) monocyte and peritoneal dialysis effluent (PDE) was examined by RT-PCR. The TNF-α and IL-6 concentrations of peripheral blood monocyte were estimated by ELISA. Results The PPAR-γ mRNA expression of peripheral blood monocyte and PDE monocyte on day 1 of EG (0.52±0.06 and 0.68±0.07) respectively was significantly lower than that of CG (0.68±0.08 and 0.93±0.06) respectively that of EG and on day 5 (0.66±0.09 and 0.89±0.08 respectively). The TNF-α and IL-6 concentrations of peripheral blood monocyte on day 1 of EG [(20.16±11.26)ng/L and (26.89±13.46)ng/L respectively] were significantly higher than that of CG [(6.38±4.28)ng/L and (8.96±4.67)ng/L respectively] and day 5 of EG [(5.39±3.50)ng/L and (7.83±4.26)ng/L respectively]. A significant negative correlation were observed between TNF-α and IL-6 concentration and PPAR γ mRNA expression. Conclusion The PPAR-γ mRNA expression was significantly low at the initial stage of peritonitis in peritoneal dialysis patients. These data indicate that the PPARγ might be involved in the defense mechanism of the peritoneum.

Key words: Peritonitis, Peroxisorne proliferator-activated receptor-γ

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