晚期氧化蛋白产物对血管平滑肌细胞单核细胞趋化蛋白-1表达的影响

彭侃夫; 吴雄飞; 赵洪雯; 孙岩; 吴亿; 王军霞

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中国血液净化 ›› 2006, Vol. 5 ›› Issue (5) : 252-255.

基础研究

晚期氧化蛋白产物对血管平滑肌细胞单核细胞趋化蛋白-1表达的影响

彭侃夫吴雄飞赵洪雯孙岩吴亿王军霞

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摘要

目的研究晚期氧化蛋白产物对血管平滑肌细胞单核细胞趋化蛋白-1表达的影响。方法采用逆转录聚合酶链反应技术和酶联免疫吸附试验测定细胞中单核细胞趋化蛋白-1mRNA的表达量及蛋白的含量。观察晚期氧化蛋白产物对平滑肌细胞单核细胞趋化蛋白-1表达的影响。结果晚期氧化蛋白产物促进平滑肌细胞单核细胞趋化蛋白-1mRNA和蛋白表达的作用( P＜0．01)。结论晚期氧化蛋白产物促进平滑肌细胞单核细胞趋化蛋白-1mRNA和蛋白表达可能是其致动脉粥样硬化机制之一。

Abstract

Objective To study the expression of monocyte chemotactic protein-1(MCP-1)mRNA and protein induced by advanced oxidation protein products(AOPP )in smooth muscle cells(SMCs)．Methods Reverse transcription polymerase chain reaction(RT-PCR)and enzyme-linked immunosorbent assay(ELISA)were adopted to measure expression of MCP-1 mRNA and protein．The effect of AOPP on the MCP-1 expression was observed．Results AOPP could enhance the expression of MCP-1 mRNA and protein inSMCs (P＜0.01)．Conclusions It is possibly one of the atherosclerotic mechanisms that AOPP enhance the expression of MCP-1mRNA and protein inSMCs.