聚苯乙烯磺酸镧治疗腺嘌呤性慢性肾衰竭动物模型钙磷代谢异常的实验研究

苗华; 洪虹; 潘明明

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中国血液净化 ›› 2007, Vol. 6 ›› Issue (5) : 272-275.

基础研究

聚苯乙烯磺酸镧治疗腺嘌呤性慢性肾衰竭动物模型钙磷代谢异常的实验研究

苗华洪虹潘明明

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聚苯乙烯磺酸镧治疗腺嘌呤性慢性肾衰竭动物模型钙磷代谢异常的实验研究

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摘要

目的观察聚苯乙烯磺酸镧对大鼠腺嘌呤性慢性肾衰竭钙磷代谢的影响。方法雄性Wastar大鼠90只，参照Yokozawa方法腺嘌呤0.3g/(kg·d)灌胃，连续25d。将造模成功动物随机分为5组，分别为聚苯乙烯磺酸镧1.500、0.750、0.375g/kg 3个剂量组，阳性对照碳酸钙咀嚼片组及模型对照组，各组大鼠每日给相应供试品，连续用药22d。另设一组空白对照组。给药开始后7、14、21d称取动物体重，观察体重变化。给药10、21d后1h，自大鼠颈静脉取血检测血清PTH、Urea、Cre、P、Ca、K、RBC、Hb、PLT、HCT。给药22d后1h处死大鼠，摘取双侧肾脏，称重肾脏系数并进行病理组织学检查(待测)，进行统计学处理。结果聚苯乙烯磺酸镧在本试验中大剂量组[1.5g/(kg·d)]可明显降低腺嘌呤性慢性肾衰竭大鼠血清P水平，并显示出一定程度的降钾作用，对其他指标无影响。阳性对照组对血清钙有明显升高作用。结论聚苯乙烯磺酸镧可有效降低腺嘌呤性慢性肾衰竭大鼠血清磷水平，并显示出一定程度的降钾作用。

Abstract

Objective To observe the effect of polystyrene sulfonic lanthanum on calcium and phosphate metabolism in rats with chronic renal failure induced by adenine. Methods Ninety male Waster rats fed with adenine 0.3g/(kg·d) for 25 days. Rats were then divided into five groups. The first, second and third groups were fed with polystyrene sulfonic lanthanum 1.5g/(kg·d), 0.75g/(kg·d) and 0.375g/(kg·d), respectively, for 22 days. Rats in the fourth group were fed with calcium carbonate and those in the fifth group without drugs as the control groups. Body weight was measured at day 7, 14 and 21 of the treatment. Serum calcium, phosphate, PTH, urea, creatinine, K+, RBC, Hb, platelet and hematocrit were determined one hour after the treatment at day 10 and 21. Rats were killed and the kidney was taken for analysis at day 22 of the treatment. Results Serum phosphate decreased significantly in rats fed with polystyrene sulfonic lanthanum 1.5g/(kg·d). Serum K+ also lowered to a certain degree in rats of this group. In the positive control group fed with calcium carbonate, serum calcium elevated. Conclusion Polystyrene sulfonic lanthanum decreases serum phosphate in rats with chronic renal failure induced by adenine. It also reduces serum K+ to a certain degree.