中国血液净化

›› 2008, Vol. 7 ›› Issue (4): 195-197.

• 基础研究 • 上一篇    下一篇

外周血单个核细胞凋亡评价血液透析膜生物相容性的实验研究

罗洋 卞维静   

  1. 卫生部中日友好医院肾病中心 卫生部中日友好医院肾病中心
  • 收稿日期:2008-01-08 修回日期:1900-01-01 出版日期:2008-04-12 发布日期:2008-04-12
  • 通讯作者: 罗洋

Apoptosis of peripheral blood mononuclear cells as an index to evaluate biocompatibility of hemodialysis membranes

LUO Yang, BIAN Wei-jing.   

  1. Center for Nephrology, China-Japan Friendship Hospital, Beijing 100029, China
  • Received:2008-01-08 Revised:1900-01-01 Online:2008-04-12 Published:2008-04-12

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摘要： 目的 探讨单个核细胞（PMBC）凋亡是否可以作为评价透析膜生物相容性的指标。方法 分别用醋酸纤维膜（CA）和聚砜膜（PS）与血液透析患者PMBC孵育不同时间（0、24、48、72小时），以流式细胞术、酶联免疫法（ELISA）检测不同透析膜对PMBC凋亡、白细胞介素-1（IL-1）及肿瘤坏死因子（TNF- ）合成的影响。结果 （1） CA膜组PMBC凋亡程度明显高于PS膜及正常对照组（P<0.05），PS膜组与正常对照之间无显著性差异（P>0.05）。（2）对PMBC凋亡OD值和IL-1及TNF- 合成量成正相关（r=0.725；r=0.813，P<0.01）。结论 生物相容性不同的透析膜对透析患者PMBC凋亡存在着不同程度的影响，PMBC凋亡程度可以作为衡量透析膜生物相容性的指标。

关键词: 血液透析, 外周血单个核细胞, 凋亡, 生物相容性

Abstract: Objective To study whether the apoptosis of peripheral blood mononuclear cells (PMBC) can be used as an index to evaluate the biocmpatibility of hemodialysis membranes. Methods PMBC of hemodialysis patients were stimulated in vitro with different biocompatibility membranes for 0 to 72 hours, then Flow cytometry and ELISA analysis were applied to evaluate the apoptosis and the synthesis of interleukin-1 and tumor necrosis factor- of PMBC. Results Bad biocompatibility membrane such as CA membrane can cause much heavier apoptosis of PMBC than good biocompatibility membrane can do, the absorbent density (OD) which represents the degree of PMBC apoptosis in CA group was significant higher than that in PS group and control group. In CA group, OD in 48 hr was significantly higher than that in 24hr and it had no significant different with OD in 72 hr (P>0.05). Our data also showed that the apoptosis of PMBC had a significant positive correlation with the production of interleukin-1 and tumor necrosis factor- during which they were stimulated. Conclusion It is advisable to use the apoptosis of PMBC as an index to evaluate biocmpatibility of hemodialysis membranes.

Key words: Peripheral mononuclear cells, Apoptosis, Biocompatibility

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