中国血液净化

›› 2009, Vol. 8 ›› Issue (12): 649-651.

• 论著 •    下一篇

血液透析患者外周血单核细胞COX-2的表达及其与炎症细胞因子水平的关系

尹忠诚 吕梅月 濮红梅   

  1. 徐州医学院附属医院肾脏内科； 徐州医学院附属第三医院肾脏内科
  • 收稿日期:2009-03-11 修回日期:1900-01-01 出版日期:2009-12-12 发布日期:2009-12-12
  • 通讯作者: 尹忠诚

COX-2 expression in mononuclear cells of peripheral blood and the relationship between COX-2 and inflammatory cytokines in hemodialysis patients

YIN Zhong-cheng, LV Mei-yue, PU Hong-mei   

  1. 1Department of Nephrology, Affiliated Hospital of Xu Zhou Medical College, Xu Zhou 221006; 2 Department of Nephrology, The Third Affiliated Hospital of Xu Zhou Medical College, Xu Zhou 221003
  • Received:2009-03-11 Revised:1900-01-01 Online:2009-12-12 Published:2009-12-12

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摘要：

【摘要】 目的 探讨血液透析患者外周血单核细胞环氧合酶-2(COX-2)的表达与炎症细胞因子IL-6、IL-8水平的关系。 方法 采用ELISA的方法测定48例维持性血液透析患者和25例健康对照组外周血单核细胞培养上清中COX-2、IL-6、IL-8水平,并测定两组观察对象血清高敏C反应蛋白(hs-CRP)的浓度。结果 血液透析患者hs-CRP、COX-2、IL-6、IL-8水平明显高于健康对照组，COX-2与IL-6、IL-8、hs-CRP之间存在相关性。结论 维持性血液透析患者存在微炎症状态，COX-2与IL-6、IL-8之间可能存在的正反馈效应促进患者微炎症状态的发展。

关键词: 维持性血液透析, 微炎症状态, 环氧合酶-2, IL-6, IL-8

Abstract:

【Abstract】 Objective To evaluate cyclooxyenase-2 (COX-2) expression in mononuclear cells of peripheral blood and the relationship between COX-2 expression and levels of inflammatory cytokines IL-6 and IL-8 in hemodialysis patients Methods We recruited 48 maintenance hemodialysis patients and 25 healthy volunteers in this study. ELISA was used to detect COX-2, IL-6 and IL-8 in the medium of cultured mononuclear cells from peripheral blood. Serum high sensitive C reactive protein (hs-CRP) was also measured. Results hs-CRP, COX-2, IL-6 and IL-8 levels were significantly higher in maintenance hemodialysis patients than in healthy controls. Correlation was found between COX-2 expression and levels of IL-6, IL-8 and hs-CRP. Conclusion Microinflammatory state presented in maintenance hemodialysis patients. Positive feedback between COX-2 expression and the levels of IL-6 and IL-8 may exist to promote the microinflammatory state in these patients.

Key words: Microinflammatory state, Cyclooxyenase-2, Interleukin-6, Interleukin -8

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