【Abstract】 Objective To determine the effect of closed-rinse on hemodialysis related eosinophilia. Methods Seven patients on regular hemodialysis and with sustained hemodialysis related eosinophilia were enrolled in this study. We used closed-rinse to clean the dialyzers and tubes for 3 months. Peripheral eosinophil count and percentage, and clinical symptoms were observed every month. Results Eosinophil count and percentage decreased significantly (median count decreased from 0.73 to 0.48, median percentage from 13.0% to 5.6%, P＜0.05) after using the closed-rinse. Conclusion Closed-rinse to clean the dialyzers and tubes may reduce the presence of hemodialysis related eosinophilia.