中国血液净化

›› 2008, Vol. 7 ›› Issue (1): 29-32.

• 论著 • 上一篇    下一篇

高糖透析液对大鼠腹膜结缔组织生长因子表达的作用

李建飞 刘伏友 刘 虹 彭佑铭 李 萍   

  1. 中南大学湘雅二医院肾内科
  • 收稿日期:2007-09-26 修回日期:1900-01-01 出版日期:2008-01-12 发布日期:2008-01-12

High glucose peritoneal dialysate on expression of connective tissue growth factor in rat peritoneum

LI Jian-fei, LIU Fu-you， LIU Hong， PENG You-ming， LI Ping   

  • Received:2007-09-26 Revised:1900-01-01 Online:2008-01-12 Published:2008-01-12

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摘要： 【摘要】目的 探讨腹膜透析液对大鼠腹膜结缔组织生长因子(CTGF)的表达及细胞外基质(ECM)合成的影响。方法 将中南大学湘雅二医院动物实验中心提供的SD大鼠随机分为4组：正常对照组(Con)、生理盐水组(NS)、低糖透析液组(LG)、高糖透析液组(HG)。4周后，评价腹膜的功能。VG染色镜下测量腹膜胶原组织厚度。免疫组织化学方法检测CTGF、TGF-β1及纤连蛋白(FN)蛋白质的表达。采用RT-PCR检测大鼠腹膜组织中CTGF和TGF-β1 mRNA的表达。结果 LG、HG组与Con、NS组相比较，超滤量均显著下降(P＜0.05)；HG组与LG组比较，其超滤量明显减少(P＜0.05)。D/Pcr的比较：HG组比Con、NS组升高(P＜0.05) ；D/DO的比较：HG组比Con、NS组明显下降(P＜0.05)，LG组比Con组低(P＜0.05)。HG组腹膜胶原厚度比与其余各组均增厚(P＜0.05)；LG组比Con组增厚(P＜0.05)；HG组CTGF、TGF-β1、FN蛋白表达水平显著高于其他各组(P＜0.05)；LG组显著高于Con、NS组(P＜0.05)。在Con及NS组中，TGF-β1、CTGFmRNA呈低水平表达，TGF-β1、CTGFmRNA在HG组中表达最高(P＜0.05)。结论 腹膜透析液尤其是高糖腹膜透析液，能明显诱导大鼠腹膜CTGF的表达增强，并促使腹膜纤维化发生。

关键词: 腹膜透析液, 结缔组织生长因子, 腹膜纤维化, 转化生长因子-β1

Abstract: 【Abstract】Objective To investigate the effect of peritoneal dialysis fluids on connective tissue growth factor (CTGF) expression and extracellular matrix synthesis in the peritoneum of peritoneal dialysis rats. Methods Sprague-Dawley rats were randomly divided into 4 groups based on the dialysate used: control group (Con),0.9% saline solution (NS) group,1.5% Dianeal (LG) group and 4.25% Dianeal (HG) group. Peritoneal function was estimated and peritoneal collagen thickness was measured 4 weeks after the treatment. Transforming growth factor beta 1 (TGF-β1), CTGF, fibronectin (FN) were detected by immunohistochemistry. RT-PCR was used to evaluate the expression of TGF-β1 and CTGF mRNAs. Results The ultrafiltration volume was lower in LG and HG groups than in Con and NS groups (P＜0.05), and was lower in HG group than in LG group (P <0.05). D/Pcr was higher in HG group than in Con and NS groups (P＜0.05). D/D0 glucose was lower in HG group than in Con and NS groups, and was lower in LG group than in Con group (P＜0.05). The collagen of peritoneal membrane was thicker in HG group than in other groups (P＜0.05), and was thicker in LG group than in Con group (P <0.05). TGF-β1 and CTGF mRNA and their proteins were higher in LG and HG groups than in Con and NS groups (P <0.05), and were significantly higher in HG group than in LG group (P <0.05). FN protein was significantly increased in HG group, as compared with that in other groups (P＜0.05), and was higher in LG group than in Con and NS groups (P <0.05). Conclusion Peritoneal dialysis fluids, especially those containing higher concentrations of glucose, up-regulates the expression of CTGF mRNA and its protein, and contributes to the peritoneal fibrosis.

Key words: Connective tissue growth factor, Peritoneal fibrosis, Transforming growth factor beta 1