关键词: 氯沙坦, 肾间质纤维化, 转分化

Abstract: Objective To investigate the effect of ATRA Losartan on rats renal interstitial cells after unilateral ureteral obstraution(UUO). Methods The UUO models were induced by ligating the right ureter.Rats were divided into normal control(sham operation), model and treatment group(Losartan 16 mg/kg/d by gastric gavage from the same operation day to day 14 after the induction of UUO). The expression of TGF-β was detected by immunohistochemical staining. E-cadherin and α-SMA was analyzed by double-labeled immuno-histochemical staining. And FITC-labledα-SMA was also observed by confocal laser scanning microscopy. Results The obstructed kidney displayed interstitial fibrotic lesions at 14 days after UUO. The TGF-βwas overexpressed（P＜0.01）, with upregulated α-SMA（P＜0.01）, and decrased E-cadherin level（P＜0.05）. Both E -cadherin and α-SMA’s were expression seen in many tubular epithelium cells. And tubular basement membrane lesion was seen in confocal microscopy. Compared with the UUO group, losartan treatment largely blunted the expression of TGF-β and α-SMA（P＜0.01）, the level of E-cadherin was upregulated. Conclusion Losartan could significantly suppress the progression of transdifferentiation of interstitial resident cells, and therefore exhibits potent therapeutic effects on renal interstitial fibrosis.

Key words: Renal interstitial fibrosis, Transdifferentiation