中国血液净化

›› 2004, Vol. 3 ›› Issue (8): 438-440.

• 基础研究 • 上一篇    下一篇

大黄素对糖尿病肾病患者外周血单个核细胞MCP-1的影响

王俭勤 王晓玲 王文革 王 静 孔玉科   

  1. 730030 兰州，兰州医学院第二附属医院肾内科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-08-12 发布日期:2004-08-12

  • Received:1900-01-01 Revised:1900-01-01 Online:2004-08-12 Published:2004-08-12

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摘要：

目的 探讨大黄素对糖尿病肾病患者外周血单个核细胞 MCP-1的影响。方法 选取32例非 胰岛素依赖型糖尿病伴肾损害患者和健康对照24例，PBMC提取采用Ficoll梯度密度离心法，细胞被分成4组，观察4种不同浓度的大黄素(0、10、50、100μg/ml)对糖尿病肾病患者外周血单个核细胞MCP-1的影响，MCP-1测定采用ELISA法。结果 糖尿病肾病外周血单个核细胞MCP-1水平高于正常对照（78.40±35.67 vs 29.30±17.31pg/ml, P<0.05），且与24h尿蛋白定量、尿白蛋白水平呈正相关关系(r=0.633，P<0.05；r=0.701， P< 0.05；)。大黄素(30～100 μg/ml)均明显降低了糖尿病肾病患者外周血单个核细胞MCP-1水平(78.40±35.67 vs 61.00±28.90，P<0.05；78.40±35.67 vs 41.00±18.90, P<0.01；78.40±35.67 vs 21.00±6.90，P<0.01)，大黄素浓度越大，抑制效应越明显（P<0.01）。结论 糖尿病肾病尿 MCP-1水平是增高的，大黄素能抑制糖尿病肾病患者外周血单个核细胞MCP-1水平，大黄素下调MCP-1的表达可能是其降低蛋白尿、改善肾功能的重要环节。

关键词: 大黄素, 外周血单个核细胞, MCP-1

Abstract:

Objective　 To investigate the effect of Emodin on the secretion of MCP-1 in peripheral blood mononuclear cells (PBMCs) of patients with diabetic nephropathy. Methods Thirty-two patients of diabetic nephropathy treated with insulin and 24 healthy control were studied. PBMCs were isolated by density gradient centrifugation with Ficoll-Paque and divided into four groups. The effect of Emodin with different density(0、10、50 and 100(g/ml) on MCP-1 secretion in peripheral blood mononuclear cells of patients with diabetic nephropathy which was examined by ELISA were observed. Results The level of MCP-1 in peripheral blood mononuclear cells of diabetic nephropathy patients were higher than that of the normal controls （78.4±35.67 vs 29.3±17.31pg/ml, P<0.05）, which were positively related to the level of urinary albumin and the serum creatinine respectively (r=0.633，P<0.05；r=0.701， P<0.05）. MCP-1 level of patients with diabetic nephropaty decreased obviously by Emodian and the decreasing degree were enhanced by the increasing of Emodin density. Conclusion Patients with diabetic nephropaty have higher MCP-1 level compared with normal controls.Emodin could downregulate the express ion of MCP-1, which probably constituted the important parts of the mechanism of Emodin functon as of decreasing urinary protein and improving renal function.

Key words: Peripheral blood mononuclear cells, MCP-1

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