中国血液净化

›› 2004, Vol. 3 ›› Issue (10): 537-539.

• 论著 • 上一篇    下一篇

PPAR-γ激动剂抑制同型半胱氨酸致人外周血单个核细胞mPGEs蛋白表达

刘 虹 彭佑铭 刘伏友 王海涛 刘映红 段绍斌   

  1. 410011 长沙，中南大学湘雅二医院肾内科(刘虹，彭佑铭，刘伏友，刘映红，段绍斌)510010 广州，广州军区广州总医院肾病科(王海涛)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-10-12 发布日期:2004-10-12

  • Received:1900-01-01 Revised:1900-01-01 Online:2004-10-12 Published:2004-10-12

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摘要：

目的 体外培养人外周血单个核细胞 (PBMC)，采用同型半胱氨酸(HCY) 和PPAR-γ激动剂 (Troglitazone和Rosiglitazone)干预，探讨HCY和PPAR-γ与膜相关前列腺素E合成酶(mPGEs) 蛋白表达之间的关系。方法 抽取正常志愿者静脉血分离PBMC并培养，分别加入不同浓度的HCY及PPAR-γ激动剂培养24小时，收集细胞培养液上清，采用ELISA测定mPGEs的水平。结果 HCY实验组mPGEs高于对照组 （P<0.05）， mPGEs水平随HCY剂量升高而升高，呈剂量依赖性（P<0.05）。 PPAR-γ激动剂对HCY100μmol/L引起mPGEs水平的升高有明显抑制作用（P<0.01）。结论 HCY 促进PBMC mPGEs蛋白表达增加；PPAR-γ激动剂对HCY引起mPGEs水平升高有抑制作用。

关键词: 同型半胱氯酸(HCY), 外周血单个核细胞, 过氧化物酶增殖体激活受体(PPAR)-γ激动剂, 膜相关前列腺素E合成酶(mPGEs)

Abstract:

Objectives To investigate the effect of homocysteine (HCY) on mPGEs. Expression of human peripheral blood mononuclear cell. Methods PBMC isolated from healthy volunteers, were cultured and intervened with HCY, Troglitazone and Rosiglitazone for 24 hours. mPGEs expression in the supernatants of cell culture were analyzed by ELISA. Results The OD value of mPGEs in HCY experimental groups were higher than in controls (P<0.05). The OD value of mPGEs increased with a HCY dosage-dependent style between 20μmol/L to 100μmol/L. All groups of Troglitazone and Rosiglita-zone significantly reduced the level of mPGEs induced by 100μmol/L HCY (P<0.01), but there was no significant difference between the two PPAR-P activator groups (P>0.05). Conclusions HCY could stimulate PBMC to express mPGEs dosage-dependently. PPAR-γactivators could inhibit PBMC releasing and expressing mPGEs induced by HCY. 

Key words: Peripheral blood mononuelear cell (PBMC), Peroxisome proliferator-activated receptorsγ (PPAR)-γ, Memrane associated prostaglandin E synthase (mPGEs)

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