中国血液净化

›› 2004, Vol. 3 ›› Issue (11): 605-608.

• 基础研究 • 上一篇    下一篇

辅助肾小管装置免疫调节机理研究

严泉剑 陈香美 吴镝 谢院生 付博 张利 李志辉 张宇梅 马志芳 卢英杰 申力军 刘秀娟 孙雪峰   

  1. 100853 北京，解放军总医院肾科，解放军肾病中心暨重点实验室
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-11-12 发布日期:2004-11-12
  • 通讯作者: 陈香美

  • Received:1900-01-01 Revised:1900-01-01 Online:2004-11-12 Published:2004-11-12

RichHTML

0

PDF

178

摘要：

目的 研究辅助肾小管装置参与全身免疫调节的机理。方法 分别用感染患者持续血液滤过(continuous venovenous hemofiltration，CVVH）超滤液和脂多糖（LPS）干预培养人肾小管上皮细胞系HKC细胞，用RT-PCR、ELISA检测IL-10转录水平和蛋白水平表达变化，用"transwell"方法检测IL-10在HKC细胞内的转位特点。结果 RT-PCR、ELISA结果显示，HKC细胞暴露于感染患者CVVH超滤液或含LPS培养液后，IL-10转录水平升高，上清中IL-10蛋白水平升高。"transwell"方法检测发现IL-10向两侧分泌。结论 辅助肾小管装置中的HKC可以通过表达IL-10，参与全身免疫调节。

关键词: 辅助肾小管装置, IL-10 免疫

Abstract:

Objective To study the immune modulation mechanism of renal tubule assist device (RAD) in end-stage renal disease (ESRD) therapy. Methods Cultured with serum of sepsis or medium contain lipopolysaccharide(LPS) on human kidney tuble epithelial cell lines HKC，the transcription and translation expression of interleukin-10(IL-10) were detected with RT-PCR、ELISA. The translocation of IL-10 in HKC stimulated with LPS was detected with "transwell". Results The expression of IL-10 showed higher in HKC from renal tubule assist device stimulated with LPS than that in normal culture medium or ultrafiltrate. The HKC can secret IL-10 toward both apical and basal. Conclusion The HKC cells in RAD take part in immune regulation with the expression of IL-10 stimulated by LPS.

Key words: Interleukin-10, End-stage renal disease

中图分类号: