中国血液净化

›› 2003, Vol. 2 ›› Issue (7): 363-365.

• 论著 • 上一篇    下一篇

血液透析患者血浆中可溶性CD40及CD4+T细胞上CD154水平的变化

李艳春 廖常志 王 莉 何 强 王 芳 杨秀川   

  1. 610072 成都，四川省人民医院肾脏科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-07-19 发布日期:2003-07-19

  • Received:1900-01-01 Revised:1900-01-01 Online:2003-07-19 Published:2003-07-19

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摘要： 目的 探讨 CD40-CD40配体（CD154）相互作用在血液透析患者免疫异常及肾脏损伤中的可能作用。方法 慢性肾功能衰竭进行血液透析患者22名，透析前采静脉血，正常对照10名，非空腹采静脉血；sCD40的测定采用ELISA法，CD4+T细胞上CD154的测定采用双色流式细胞仪。结果 sCD40在正常对照血浆中水平为（30.28±11.09）pg/ml，而血液透析患者为（781.22±203.03）pg/ml，显著高于正常对照( P<0.01)。外周血 CD4+T细胞上CD154在正常对照的水平为（29.24±3.99）%，而血液透析患者为（41.41±5.54）%，也明显高于正常对照 (P<0.05)；说明在血液透析患者，其 CD40-CD154相互作用处于过度活跃状态。 结论 CD40-CD154可能通过影响免疫功能及加速肾脏损伤参与了尿毒症的发生发展，阻断CD40-CD154相互作用可能有助于延缓疾病进程。

关键词: 血液透析, CD40, CD40配体

Abstract:

Objective To investigate potential mechanisms by which CD40-CD40 ligand (CD40L, CD154) interaction may be involved in the pathogenesis of immunodeficiency and renal injury in haemodialysis patients. Methods Soluble CD40 and CD154 on CD4+T cells was detected in the serum of haemodialysis patients (n=22) and healthy donors (n=10) by the method of ELISA and two color flow cytometry, respectively. Results Soluble CD40 was detected in the serum of haemodialysis patients with a mean of(781.22± 203.03)pg/ml，significantly higher than healthy donors(30.28±11.09)pg/ml, P<0.01; CD154 on CD4+T cells was detected in the serum of haemodial ysis patients with a mean of （41.41±5.54）%，elevated comparing with healthy donors (29.24±3.99)%, P<0.05. CD40-CD40 ligand (CD40L) interaction may be actiatved in haemodialysis patients. Conclusion CD40-CD154 may be involved in the pathogenesis of uremia by the means of immunodeficiency and renal injury.

Key words: CD40, CD40 ligand