中国血液净化

›› 2003, Vol. 2 ›› Issue (8): 436-438.

• 基础研究 • 上一篇    下一篇

自制含不同磷、钙量的大鼠实验饲料的研究

王小兵 王笑云 周富华   

  1. 210029 王笑云，南京医科大学第一附属医院
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-08-19 发布日期:2003-08-19

  • Received:1900-01-01 Revised:1900-01-01 Online:2003-08-19 Published:2003-08-19

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摘要： 目的 探索自制适合磷代谢、继发性甲状旁腺功能亢进研究的含不同磷、钙剂量的大鼠饲料。 方法 适当调整 AIN-93G标准配方，自制含不同磷、钙剂量饲料并检测鉴定含量，然后分别用高、低磷饲料喂饲慢性肾衰竭（CRF）和假手术对照大鼠2周；分别测定血离子钙(iCa)、磷(P)、甲状旁腺激素(iPTH)、活性维生素D3。结果 高磷饲料含磷1.2%、钙1.6%，低磷饲料含磷0.2%、钙0.5%；喂饲大鼠2周后，高磷CRF组较低磷CRF组血磷(3.93±0.27) vs (2.42±0.84)mmol/L, P<0.05)及 iPTH(75.50±20.19) vs (30.55±2.33), P<0.05)明显上升,两组血离子钙(1.15±0.08) vs (1.20±0.08)mmol/L, P>0.05)及活性维生素 D3(69.77±10.85) vs (87.51±8.63)pg/ml, P>0.05)无明显差异。结论 本配方能提供慢性肾衰竭磷代谢实验研究所需的高、低大鼠磷饲料，具有应用和推广价值。

关键词: 大鼠饲料, 磷, 钙

Abstract: Objective To study the laboratory rat's feeds with different phosphorus(P) and calcium(Ca) contents for research on the metabolism of P. Methods AIN-93G formulat was modified partly to manufacture the feeds with different P and Ca contents. The high-P and low-P feeds were fed to the CRF rats or sham operation control rats respectively for two weeks.The levels of serum ionized calcium,phosphorus, iPTH and 1,25-(OH)2D3 were observed. Results The high-P diet contains 1.2% P and 1.6% Ca,and the low one contains 0.2% P and 0.5% Ca. After two weeks, serum P and iPTH levels in CRF rats fed with high-P feeds were significantly higher than those of CRF rats fed with low-P feeds (P 3.93±0.27 vs 2.42±0.84 mmol/L, P<0.05; PTH 75.50 ± 20.19 vs 30.55 ± 2.33pg/ml, P<0.05, respectively), but there were no significant difference in serum ionized calcium and 1,25-(OH)2D3. Conclusion The formula of feeds for the laboratory rats in this experiment can fit for the requirement of research on the phosphorus metabolism. 

Key words: Phosphorus, Calcium