中国血液净化

›› 2002, Vol. 1 ›› Issue (7): 3-6.

• 论著 • 上一篇    下一篇

使用不同透析膜时IL-8表达的改变

吕 路 黄锋先 张增华 桓文穆 黄 华   

  1. 510080 广州，广州铁路中心医院肾内科(吕路，张增华，桓文穆，黄 华) 510089 广州，中山大学附属第一医院肾内科(黄锋先)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-07-19 发布日期:2002-07-19

  • Received:1900-01-01 Revised:1900-01-01 Online:2002-07-19 Published:2002-07-19

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摘要： 【摘要】目的 解不同透析膜对血液透析患者IL-8表达的影响。方法 采用酶联免疫吸附试验（ELISA）测定IL-8血浆水平及外周血单个核细胞（PBMC）培养上清液IL-8水平，逆转录多聚酶链反应（RT-PCR）检测基因表达。结果 聚砜膜组的IL-8血浆水平及mRNA表达低于铜仿膜组及铜氨膜组，而铜仿膜组及铜氨膜组之间无显著差异。各组透析后的IL-8血浆水平及mRNA表达均较透析前升高。铜仿膜组透析前PBMC受LPS刺激后产生的IL-8多于聚砜膜组，血透后聚砜膜组产生的IL-8较透析前升高，而铜仿膜组较透析前无明显改变。结论 再生纤维素膜（尤其是铜仿膜）导致IL-8表达改变的能力强于聚砜膜，测量IL-8表达有助于评价不同透析膜的生物相容性。

关键词: 血液透析, 白细胞介素8(IL-8), 透析膜, 尿毒症

Abstract:
【Abstract】Objective To investigate the influence of the different dialysis membrane on the interleukin-8 expression in uremic patients. Methods The plasma concentration of IL-8 and the IL-8 production by the cultivated PBMC were measured with enzyme-linked immunoabsorbent assay(ELISA). The gene expression of IL-8 was measured with reverse transcription-polymerase chain reaction(RT-PCR). Results The IL-8 plasma and mRNA level of the patients using the regenerated cellulose membrane (cuprophan CU, cuprammonium rayon CR) is higher than that of those using polysulfone (PS) membrane. The IL-8 plasma level and mRNA expression increases after hemodialysis. The production of IL-8 from PBMC of the patients using CU membrane was more than that of those using PS membrane. In PS group the IL-8 production of PBMC increased after dialysis. But there was no obvious difference of IL-8 production from PBMC between pre-dialysis and post-dialysis in CU group. Conclusion The change of IL-8 mRNA expression and plasma level through the hemodialysis in the regenerated cellulose membrane (especially CU) groups is more significant than that in PS group. The measurement of IL-8 expression is useful to evaluate the biocompatibility of dialysis membranes.

Key words: Interleukin-8(IL-8), Dialysis membrane, Uremia