中国血液净化

›› 2009, Vol. 8 ›› Issue (2): 88-91.

• 基础研究 • 上一篇    下一篇

罗格列酮对人腹膜间皮细胞和脐静脉内皮细胞水孔蛋白-1的影响

黄丛洋 张小娟 姚 强 戴慧莉 倪兆慧? 钱家麒

  

  1. 上海交通大学医学院附属仁济医院肾脏科
  • 收稿日期:2008-11-22 修回日期:1900-01-01 出版日期:2009-02-12 发布日期:2009-02-12

Effect of rosiglitazone on aquaporin-1 expression in human peritoneal mesothelial cells and human umbilical vein endothelial cells

HUANG Cong-yang, ZHANG Xiao-juan, YAO Qiang, DAI Hui-li, NI Zhao-hui, QIAN Jia-lin..   

  1. Department of Nephrology, Shanghai Renji Hospital affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200001
  • Received:2008-11-22 Revised:1900-01-01 Online:2009-02-12 Published:2009-02-12

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摘要： 【摘要】目的 探讨体外条件下核受体Peroxisome proliferator-activated receptor gamma(PPAR-gamma)激动剂罗格列酮(Rosiglitazone RGZ)对人腹膜间皮细胞(human peritoneal mesothelial cells HPMCs)、人脐静脉内皮细胞( Human Umbilical Vein Endothelial Cells HUVECs)水孔蛋白-1(aquaporin 1 AQP1）增殖以及基因水平的影响。方法 以HPMCs和HUVECs为研究对象，将实验分成四组：对照组、RGZ组(10uM RGZ)、GW9662组(peroxisome proliferator-activated receptor gamma antagonist 20uMGW9662)以及GW9662+R组(20uMGW9662提前作用1小时后加入10uMRGZ)。应用比色法(CCK-8)观察RGZ等对HPMCs和HUVECs增殖的影响。应用 Real time桺CR方法检测罗格列酮等对水孔蛋白-1基因水平表达的影响。结果 ①GW9662+R组与对照组相比,可以明显抑制HPMCs增殖(P＜0.01)，其他组对HPMCs增殖没有明显影响(P＞0.05)。而对HUVECs，RGZ组、GW9662组以及GW9662+R组都可以促进其增殖(P＜0.01)； ②罗格列酮上调这两种细胞AQP1基因水平的表达。结论 在体外，罗格列酮可影响HPMCs和HUVECs水孔蛋白质1基因水平的表达。其作用机制可能是通过激活PPAR-gamma从而影响上述两种细胞的AQP1的表达。

关键词: 罗格列酮, 间皮细胞, 内皮细胞, 水孔蛋白-1

Abstract: 【Abstract】Objective To study the effect of rosiglitazone (RGZ), a ligand of peroxisome proliferator-activated receptor γ (PPAR-γ), on the cell-proliferation and expression of aquaporin-1 mRNA in human peritoneal mesothelial cells (HPMCs) and human umbilical vein endothelial cells (HUVECs) in vitro. Methods Each kind of cells were divided into 4 groups: the control group, the RGZ group (10μM RGZ), the GW9662 group (GW9662, a PPAR-γ antagonist, 20μM) and the GW9662+RGZ group (cells were cultured for 1 h in the medium containing 20μM GW9662 and then in the medium containing 10μM RGZ). Cell-proliferation affected by RGZ was assayed by using a CCK-8 cell-counting kit. Aquaporin-1 mRNA in HPMCs and HUVECs was measured by quantitative real-time PCR. Results Proliferation of HPMCs was inhibited in the GW9662+RGZ group (P<0.01) as compared with that of the control group. The inhibition was not found in the RGZ group and the GW9662 group (P>0.05). However, proliferation of HUVECs presented in the 3 treated groups (P<0.01). RGZ up-regulated aquaporin-1 mRNA in HPMCs and HUVECs. Conclusion RGZ can up-regulate the expression of aquaporin-1 at the mRNA level, probably through the activation of the PPARγ pathway in HPMCs and HUVECs.

Key words: Mesothelial cells, Endothelial cells, Aquaporin-1