中国血液净化

›› 2011, Vol. 10 ›› Issue (3): 149-153.doi: 10.3969/j.issn.1671-4091.2011.03.00

• 基础研究 • 上一篇    下一篇

脂多糖对大鼠腹膜间皮细胞白细胞介素15、6及丙二醛表达的影响

彭 霞 马健飞 李程程 樊 怡 霍爱晶   

  1. 中国医科大学附属第一医院肾内科
  • 收稿日期:2010-05-04 修回日期:1900-01-01 出版日期:2011-03-12 发布日期:2011-03-12
  • 通讯作者: 马健飞

The effects of lipopolysaccharide on the expression of inflammatory factors interleukin 15 and interleukin 6 and malondialdehyde in rat peritoneal mesothelial cells

PENG Xia, MA Jian-fei, LI Cheng-cheng, FAN Yi, HUO Ai-jing   

  1. Department of Nephrology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China
  • Received:2010-05-04 Revised:1900-01-01 Online:2011-03-12 Published:2011-03-12

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摘要： 目的 研究脂多糖(lipopolysaccharide,LPS)对大鼠腹膜间皮细胞(rat peritoneal mesothelial cells,RPMC)白细胞介素15(interleukin-15,IL-15)、IL-6及丙二醛(malondialdehyde,MDA)的影响。 方法 胰蛋白酶法行RPMC的原代培养和传代, 经鉴定第3代用于试验。分组：①正常对照组；②LPS组：不同浓度LPS组(1 mg/L、10 mg/L、100 mg/L)分别作用6 h和12 h；③不同时间组：10 mg/L LPS分别作用于RPMC 3,6,12,24 h。实时定量PCR法测IL-15 mRNA的表达；ELISA法检测细胞培养上清液中IL-15和IL-6的表达；硫代巴比妥法测MDA。 结果 LPS可刺激RPMC的IL-15 mRNA及蛋白的表达增高且在12 h内呈时间剂量依赖性(P＜0.05)；LPS诱导RPMC IL-6的蛋白表达增高(P＜0.05)；LPS诱导RPMC MDA的含量表达增高且呈浓度依赖(P＜0.05)。 结论 IL-15作为炎症因子参与了腹膜透析相关性腹膜炎的病理过程。LPS可上调RPMC IL-15、IL-6和MDA的表达，导致腹膜炎症及氧化应激。

关键词: 脂多糖, 腹膜间皮细胞, 腹膜透析, 白细胞介素15, 白细胞介素6, 丙二醛

Abstract: 【Abstract】 Objective To observe the effects of lipopolysaccharide (LPS) on the expression of inflammatory factors interleukin 15 (IL-15) and interleukin 6 (IL-6) and malondialdehyde (MDA) in rat peritoneal mesothelial cells (RPMCs). Methods RPMCs were isolated and cultured using trypsin, and then identified. RPMCs at passage 3 were used in the experiment. The cells were divided into (i) normal control group; (ii) LPS dose/time group, LPS 1mg/L, 10mg/L or 100mg/L treated for 6 hours or 12 hours; (iii) LPS time group, LPS 10mg/L treated for 3, 6, 12 and 24 hours. IL-15 mRNA was measured by real-time RT-PCR. IL-15 and IL-6 were assayed by ELISA. MDA were determined by thiobarbituric acid method. Results IL-6, IL-15 and its mRNAs, and MDA were significantly increased in a dose-dependent manner and/or a concentration-dependent manner in the groups treated with LPS (p<0.05, compared with those of the control group). Conclusion IL-15 may be involved in the pathological processes of peritoneal dialysis-related peritonitis. LPS can up-regulate the expression of IL-15, IL-6 and MDA in RPMCs, possibly relating to the peritonitis and oxidative stress.

Key words: Peritoneal mesothelial cell, Peritoneal dialysis, Interleukin 15, Malondialdehyde, Interleukin 6