【Abstract】 Objective In order to avoid peritomum injury and to improve the efficiency of peritoneal dialysis during the dialysis, We investigated the regulating effect of protein kinase C（PKC）on Peritoneal Mesothelial Cells (PMCs) of the plasma membrane cultivated in high concentrations of glucoses. Methods PMCs that had been harvested from male Wistar rats by Enzymatic disaggregation method was cultivated in different concentrations glucoses. PKC inhibitor G6976 was applied to obstruct the functions of PKC. Indirect immunofluorescent staining was used for the identification of expression of GLUT1 on the plasma membrane. Flow cytomety was used for the analyses the mean fluorescence intensity (MFI) of GLUT1 on the plasma membrane. Glucose concentrations were examined by biochemistry analyzer. Results Compare with the control group High glucose can promote the expression of GLUT1 on PMCs, The glucose absorption of PMCs increase also (p＜0.05), and G6976 can significantly restrain both of these two effects. Conclusion High glucose up-regulated the expressions of GLUT1 on the plasma membrane in dose-dependent manner, and the absorption of glucose in PMCs increased also. The PKC signaling pathway play an important role on it, and the inhibitor of PKC can partly block the expression of GLUT1 and the transportation of glucose.