中国血液净化

›› 2008, Vol. 7 ›› Issue (6): 321-324.

• 基础研究 • 上一篇    下一篇

慢性肾衰竭大鼠主动脉UPP组分表达变化

冯 兵 杨 旭 张耀全 叶自林 袁发焕 杨惠标   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-06-12 发布日期:2008-06-12
  • 通讯作者: 冯兵

Expression of ubiquitin-proteasome components in the aorta of rats with chronic renal failure

FENG Bing, YANG Xu, ZHANG Yao-quan, YE Zi-lin, YUAN Fa-huan, YANG Hui-biao   

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-06-12 Published:2008-06-12

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摘要： 【摘要】目的 探讨慢性肾衰竭大鼠主动脉泛素-蛋白酶体组分表达变化及意义。方法 采用单肾切除、对侧肾动脉部分结扎法建立慢性肾衰竭模型，RT-PCR检测Ub、E2 mRNA表达水平，免疫组织化学检测Ub蛋白表达，免疫印迹法测定泛素化蛋白含量，并用图像分析定量。 结果 与正常大鼠比较，慢性肾衰竭大鼠术后4个月和6个月时主动脉Ub mRNA(灰度值)分别为(1.436±0.142)、(1.480±0.124)，E2 mRNA(灰度值)分别为(2.253±0.689)、(2.002±0.383)，均显著升高(P＜0.01)。与正常大鼠比较，慢性肾衰竭大鼠主动脉Ub蛋白表达(积分光密度)在4个月和6个月时分别为 (8.20± 1.15)、(9.48±2.80)，也显著升高(P＜0.01)。而慢性肾衰竭大鼠主动脉泛素化蛋白含量在术后4个月和6个月时分别为(积分光密度)(1.46±0.26)、(2.32±0.83)，均显著低于正常大鼠(P＜0.01)。蛋白酶体抑制剂MG-132可明显抑制慢性肾衰竭大鼠主动脉Ub和E2 mRNA和蛋白质表达，泛素化蛋白积聚增多。结论 慢性肾衰竭大鼠主动脉泛素-蛋白酶体信号通路显著活化。

关键词: 慢性肾衰竭, 主动脉, 泛素(Ub), 泛素活化酶(E2), 泛素化蛋白

Abstract: Objective To investigate the expression of ubiquitin-proteasome components in the aorta of rats with chronic renal failure. Methods The animal model was established by partial ligation of renal pedicle artery at one side and nephroectomy at the opposite side. Ub and E2 mRNA was surveyed by RT-PCR, Ub protein was detected by immunohistochemistry, and the amount of ubiquitinated protein was surveyed by western blot. Results In the aorta of rats with chronic renal failure, Ub mRNA increased significantly at fourth month (1.436+ 0.142) and at sixth month (1.480+0.124) after operation (P<0.01, as compared with those of the control group), E2 mRNA also increased significantly at fourth month (2.253+0.689) and at sixth month (2.002+0.383) after operation (P<0.01, as compared with those of the control group), and Ub protein increased to 8.20+1.15 at fourth month and 9.48+2.80 at sixth month (P<0.01, as compared with those of the control group). Conversely, the amount of ubiquitinated protein decreased significantly to 1.46+0.26 at fourth month and to 2.32+0.83 at sixth month (P<0.01, as compared with those of the control group). The mRNA and protein expression of Ub and E2 in the aorta were significantly inhibited by the treatment of a proteasome inhibitor MG-132, and as a result, ubiquitinated protein increased significantly in the aorta. Conclusion The ubiquitin-proteasome pathway is activated in the aorta of rats with chronic renal failure.

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