中国血液净化

中国血液净化 ›› 2013, Vol. 12 ›› Issue (04): 222-224.

• 血液净化中心管理与技术 • 上一篇    下一篇

血液透析中心环境清洁水平的监测和改善

唐钦妹1,郭淑霞2,黄文碧1,叶亚莉1   

  1. 1. 福建省福州市第二医院
    2. 福建医科大学附属第一医院肾内科
  • 收稿日期:2012-11-13 修回日期:2012-11-15 出版日期:2013-04-12 发布日期:2013-07-01
  • 通讯作者: 唐钦妹 E-mail:tqm_vip@163.com

MONITORING AND EVALUATION ON THE ENVIRONMENTAL CLEAN LEVEL OF HEMODIALYSIS ROOM

  • Received:2012-11-13 Revised:2012-11-15 Online:2013-04-12 Published:2013-07-01

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摘要： 摘要：目的 通过ATP荧光监测法和微生物培养法去评价血液透析中心环境清洁程序改进前后的清洁水平变化情况，为血液透析中心环境清洁和监测提供一个实时有效的方法。方法 选择透析室五个环境位点，通过ATP荧光监测法和微生物培养法去监测五个位点的清洁水平，通过改进现有的清洁方式再次进行监测并比较两次监测结果，评价改进后的清洁程序。结果 改进前五个监测位点ATP测量均值为788、310、530、600和900RLU，改进后清洁程序ATP测量均值176、130、120、220和239RLU。改进前微生物培养法监测均值为12、4、9、6和8cfu，改进后均值为2、1、1、1和2cfu。ATP荧光检测法和微生物培养法都表明有效改进清洁程序能够有效降低血液透析中心环境表面的微生物污染情况，ATP荧光检测法和常规细菌培养法监测清洁度变化趋势相同。结论 有效的清洁程序能够显著降低医院环境表面的污染情况，选择ATP荧光监测法能够更快速有效地评价医院环境水平。

关键词: ATP荧光监测法, 微生物培养法, 血液透析中心, 清洁

Abstract: Abstract：Objective Adenosine triphosphate (ATP) bioluminescence and microbiological culture methods were used for evaluation of cleaning procedure changed before or after in hemodialysis room. These provide a real-time and effective method on monitoring and evaluation of the environmental clean level of hemodialysis room. Methods Choose five sites from the hemodiaylsis room and use ATP bioluminescence and microbiological culture methods to monitor and record the clean level of those sites before and after changing clean procedure, comparison of two results and evaluation of a new clean procedure. Results The mean of ATP bioluminescence method before changing clean procedure respectively is 788. 310. 530. 600 and 900RLU, after cleaning procedure changed is 176. 130. 120. 220 and 239RLU.The mean of microbiological culture method respectively is 12. 4. 9. 6 and 8cfu, after cleaning procedure changed is 2. 1. 1. 1 and 2cfu. The surface contaminated level of homedialysis room was significantly reduced, the monitoring results of ATP bioluminescence are similar with microbiological culture method. Conclusions Effectively surface cleaning procedure can dramatically reduced the surface contaminated condition, ATP bioluminescence is much more quick and efficient technology used in monitoring and evaluation on the nosocomial environmental clean level.

Key words: Adenosine triphosphate (ATP) bioluminescence, microbiological culture, hemodiaylsis room, clean