中国血液净化

›› 2002, Vol. 1 ›› Issue (12): 12-15.

• 论著 • 上一篇    下一篇

活性氧在高糖诱导人腹膜间皮细胞产生IL-8、MCP-1中的作用

唐知还 张 政 刘 军 张树俭 姚 建   

  1. 200080 上海，上海市第一人民医院肾内科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-12-19 发布日期:2002-12-19

  • Received:1900-01-01 Revised:1900-01-01 Online:2002-12-19 Published:2002-12-19

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摘要： 【摘要】目的 研究高糖对培养的人腹膜间皮细胞(human peritoneal mesothelial cells, HPMC）产生趋化蛋白IL-8(白介素-8)、MCP-1（单核细胞趋化蛋白-1）的影响以及内源性活性氧（过氧化氢）在其中的作用。 方法 分别用不同浓度的葡萄糖、高渗甘露醇、葡萄糖加抗氧化剂（丙酮酸）刺激细胞，用半定量RT-PCR的方法测定趋化蛋白的基因水平，用ELISA的方法测定蛋白水平。结果 高糖可以使HPMC产生IL-8、MCP-1增加，且呈浓度依赖性，而高渗甘露醇的作用则不明显；高糖中加入抗氧化剂（丙酮酸）后，IL-8的表达下降，而MCP-1的表达未见明显的改变。 结论 高糖可以使HPMC表达IL-8、MCP-1增加，从而在透析液生物不相容性及腹膜纤维化中起作用；在IL-8的产生中，内源性的活性氧可以作为信号传导分子，起到一定的作用。丙酮酸能够通过对抗内源性氧化应激，对HPMC具有保护作用。

关键词: 腹膜透析, 人腹膜间皮细胞, 过氧化氢, 趋化蛋白

Abstract: 【Abstract】 Objective To study the effects of glucose (0.1%、3.86%) on the production of IL-8 and MCP-1 in human peritoneal mesothelialcells（HPMC） in vitro and the role of endogenous reactive oxygen species (ROS) in this course. Methods The glucose (0.1%、3.86%) with or without hydrogen peroxide (0.1mmol/L、0.5mmol/L) 、3.86%mannitol、 glucose(3.86%) with pyruvate (35mmol/L) were used in culture medium to stimulate the HPMC. IL-8(Interlukin-8)mRNA and MCP-1(Monocyte chemoattractant protein-1)mRNA were measured by semiquantative RT-PCR, moreover, the protein level of IL-8 by ELISA. Results The glucose enhanced the production of IL-8 and MCP-1 following the increasing of concentration. On the contrary, the high osmolality mannitol had no these effects； Adding the antioxidant (pyruvate) in the glucose(3.86%), the expression of IL-8 was lowered, but not for the MCP-1. Conclusion The high glucose increases the expression of IL-8 and MCP-1, which may make a role in the uncomplicabality of dialysate and fibrosis of peritoneal membrane； The endogenous ROS can induce the expression of IL-8 as a signal message. Pyruvate can protect the HPMC by counterworking the endogenous oxidant stress.

Key words: Human peritoneal mesothelial cells(HPMC), Reactive oxygen species(ROS), Chemoattractant protein