中国血液净化

中国血液净化 ›› 2013, Vol. 12 ›› Issue (12): 686-689.doi: 10.3969/j.issn.1671-4091.2013.12.00

• 基础研究 • 上一篇    下一篇

Klotho蛋白在继发性甲旁亢甲状旁腺细胞弥漫性增生与结节性增生中的不同表达探讨

张建荣1,孙长丽2   

  1. 1. 武警总医院肾内科
    2. 北京武警总医院
  • 收稿日期:2013-10-12 修回日期:2013-09-16 出版日期:2013-12-12 发布日期:2013-12-03
  • 通讯作者: 张建荣zhangjr0317@126.com E-mail:zhangjr0317@126.com

Difference expression of Klotho in parathyroid cells with nodular hyperplasia and diffusible hyperplasia

  • Received:2013-10-12 Revised:2013-09-16 Online:2013-12-12 Published:2013-12-03

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摘要： 【摘要】 目的 探讨Klotho蛋白在慢性肾衰竭继发性甲状旁腺功能亢进（SHPT）甲状旁腺细胞不同增生类型中表达的意义。方法 选取在我院肾内科住院行甲状旁腺全切术的SHPT患者50例为SHPT组，尸体甲状旁腺组织供体8例为健康对照组，收集其血清及甲状旁腺组织标本，SHPT组甲状旁腺组织按病理表现分为结节性增生组和弥漫性增生组，检测血成纤维细胞生长因子-23（FGF-23）及甲状旁腺激素（PTH）水平，免疫组化检测甲状旁腺组织中Klotho蛋白及增殖指标Ki67的表达，计算阳性细胞率。结果 （1）SHPT患者血FGF-23[(1316.80±375.15)pg/ml]与PTH[(2106.00±806.78)pg/ml]呈正相关（r=0.438，P=0.001）。（2）SHPT患者血FGF-23与甲状旁腺组织中Klotho的表达呈负相关（r=-0.379，P=0.007），血PTH与Klotho蛋白呈负相关（r=-0.361，P=0.01）。（3）SHPT组甲状旁腺细胞中Ki67的表达显著高于对照组（F=54.417，P=0.000），Klotho蛋白的表达显著低于对照组（F=49.243，P=0.000）；SHPT组结节性增生甲状旁腺组织中Ki67的表达显著高于弥漫性增生组（t=3.760，P=0.001），Klotho蛋白的表达显著低于弥漫性增生组（t=6.039，P=0.000）。结论 结节性增生甲状旁腺组织中Klotho蛋白表达低于弥漫性增生组织可能是导致SHPT患者甲状旁腺呈不同增生方式的重要原因。

关键词: 继发性甲状旁腺功能亢进, 成纤维细胞生长因子-23, Klotho蛋白

Abstract: 【Abstract】Objective To investigate the difference expression of Klotho in parathyroid cells with nodular hyperplasia and diffuse hyperplasia from Secondary Hyperparathyroidism(SHPT) patients. Method A total of 50 SHPT patients and 8 cadaver were selected were selected and accepted parathyroidectomy. SHPT patients were divided into diffusible hyperplasia group and nodular hyperplasia group according to pathology. Serum fibroblast growth factor-23(FGF-23) and parathyroid hormone(PTH) were detected. The expression of Klotho and Ki67 in different hyperplasia parathyroid cells were detected by immunohistochemistry. Results (1)In SHPT patients, serum FGF-23 was positively correlated with PTH（r=0.438，P=0.001）; (2)Serum FGF-23 was negatively correlated with the expression of Klotho in parathyroid cells（r=-0.379，P=0.007）; serum PTH was negatively correlated with the expression of Klotho in parathyroid cells（r=-0.361，P=0.01）; (3) Compared with control group, the expression of Ki67 in SHPT group was increased significantly（F=54.417，P=0.000）, and the expression of Klotho decreased significantly（F=49.243，P=0.000）. Compared with diffusible hyperplasia group, the expression of Ki67 in nodular hyperplasia increased significantly（t=3.760，P=0.001）, the expression of Klotho decreased significantly（t=6.039，P=0.000）. Conclusion Klotho may contributed to different hyperplasia of parathyroid cells in SHPT patients

Key words: Secondary Hyperparathyroidism, Fibroblast growth factor-23, Klotho