中国血液净化

中国血液净化 ›› 2015, Vol. 14 ›› Issue (10): 612-616.doi: 10.3969/j.issn.1671-4091.2015.10.00

• 基础研究 • 上一篇    下一篇

诱变乳酸杆菌对肾衰竭大鼠血肌酐、尿素浓度及肾脏病理的影响

王芳1,蒋云生2   

  1. 1. 宁波市医疗中心李惠利医院肾内科
    2. 中南大学湘雅二医院肾病研究所
  • 收稿日期:2015-05-11 修回日期:2015-07-02 出版日期:2015-10-12 发布日期:2015-10-12
  • 通讯作者: 蒋云生 E-mail:jiangys2005@sohu.com
  • 基金资助:

    国家自然科学基金资助(81270853)

The influence of mutagenesis lactobacilli on serum creatinine and urea nitrogen concentrations and renal pathology in renal failure rats

Wang fangFang 2   

  • Received:2015-05-11 Revised:2015-07-02 Online:2015-10-12 Published:2015-10-12

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摘要： 目的利用大鼠5/6 肾切除模型，探讨诱变乳酸杆菌经胃肠道清除肌酐(Cr)、尿素氮(UN)的能力及其对肾脏纤维化相关因子表达和肾脏病理的影响。方法雄性6 周龄SD 大鼠60 只随机分为4组：假手术组，模型组，原始乳酸杆菌治疗组，诱变乳酸杆菌治疗组。给药8 周后，收集24h 尿液，留取血标本，处死大鼠，收集消化液及残肾组织，测定尿、血及消化液中Cr、UN 浓度。HE 和Masson 染色观察肾脏病理变化，免疫组化检测肾组织TGF-β1 及FN 的表达。结果诱变乳酸杆菌治疗组与模型组及原始乳酸杆菌治疗组比较，尿Cr、UN 水平与模型组比较无明显变化(P＞0.05)，血和消化液Cr、UN 水平明显下降(P＜0.01)，肾小管损伤与肾间质纤维化明显减轻，TGF-β1 和FN 表达明显下降(P＜0.05)。结论诱变乳酸杆菌可能通过降解胃肠道小分子物质，显著降低慢性肾衰竭大鼠血Cr、UN 水平，降低TGF-β1 及FN在肾脏的表达，减轻肾脏间质损伤及纤维化。

关键词: 5/6肾切除, 乳酸杆菌, 肌酐, 尿素氮, 纤维化

Abstract: Objectives Using the 5/6 nephrectomy rat as an animal model, we explored the capacity of creatinine and urea nitrogen removal of mutagenesis lactobacilli via gastrointestinal tract and its effect on renal pathology. Methods Sixty SD rats were randomly divided into 4 groups: sham group, model group, lactobacilli (LB) group, and mutagenesis lactobacilli (mut-LB) group. Rats were undergone 5/6 nephrectomy except the rats in sham group. Eight weeks after the surgery, 24h-urine, orbital blood and digestive juice were collected to determine creatinine (Cr) and urea nitrogen (UN) concentrations. Pathological changes of nephridial tissue were examined by HE and Masson staining. The expressions of TGF-β1 and FN in nephridial tissue were detected by immunohistochemistry. Results In mut-LB group, urinary Cr and UN levels had no significant changes (P＞0.05), serum and digestive Cr and UN levels decreased significantly (P＜0.01), renal tubular injury and renal interstitial fibrosis reduced significantly, and TGF-β1 and FN expressions decreased (P＜ 0.05), as compared with those in sham group and LB group. Conclusion Mutagenesis lactobacilli can decrease serum Cr and UN levels, reduce the expressions of TGF-β1 and FN in renal tissue, and alleviate renal interstitial injury and fibrosis, probably through the decomposition of small molecules in the gastrointestinal tract.

Key words: 5/6 nephrectomy, Lactobacillus Bacteria, Creatinine, Urea Nitrogen, Fibrosis