中国血液净化

›› 2008, Vol. 7 ›› Issue (8): 424-426.

• 基础研究 • 上一篇    下一篇

葡萄糖降解产物对内皮细胞AQP1及eNOS表达影响研究

林星辉 钱家麒 倪兆慧 Alessandro Amore Rosanna Coppo   

  1. 200001 上海，1上海交通大学医学院附属仁济医院肾内科 2. 意大利都林大学Regina Margherita 医院肾内科
  • 收稿日期:2008-05-14 修回日期:1900-01-01 出版日期:2008-08-12 发布日期:2008-08-12

ffect of glucose degradation products on AQP1 and eNOS expression in cultured endothelial cells

LIN Xing-hui1, QIAN Jia-qi1, NI Zhao-hui1, Alessandro Amore2, Rosanna Coppo   

  1. 1Renal Division, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200001, China; 2Nephrology, Dialysis and Transplantation Department, Regina Margherita Hospital, University of Turin, Turin, Italy
  • Received:2008-05-14 Revised:1900-01-01 Online:2008-08-12 Published:2008-08-12

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摘要： 目的 研究腹膜透析液毒性成份之一 -葡萄糖降解产物(GDPs)对内皮细胞水孔蛋白1(AQP1)及eNOS表达影响。方法 选用两种相关的GDPs-2-糠醛(Fur)及甲基乙二醛(MGly)，采用与传统腹膜透析液浓度相当的GDPs刺激内皮细胞系t End.1 24h(Fur 0.8 Um； MGly 35 uM)，以培养液DMEM作为对照组，研究GDPs对内皮细胞AQP1及eNOS表达影响。并进一步通过时间依赖性及浓度依赖性分析研究GDPs对AQP1 mRNA表达影响。使用RT-PCR检测AQP1及eNOS基因表达。使用Western blot检测AQP1蛋白表达。 结果 内皮细胞系t End.1经GDPs(Fur 0.8 uM；MGly 35 uM)刺激24h后，Fur及MGly显著上调内皮细胞eNOS mRNA表达(P＜0.05)。GDPs对AQP1 mRNA表达有降低的趋势但无统计学意义。时间依赖性及浓度依赖性研究同样提示GDPs对AQP1 mRNA表达有下降的趋势。Western blot检测有相似的结果。结论 GDPs促进内皮细胞eNOS的表达但并不促进AQP1的表达，GDPs的这种作用可能参与长期腹膜透析过程中腹膜血管新生及超滤衰竭的发生。

Abstract: Objective To analysis the effect of glucose degradation products (GDPs) on aquaporin-1(AQP1) and endothelial nitric oxide synthase (eNOS) expression in cultured endothelial cells. Methods Endothelial cell line (t End.1) was incubated for 24 hours with two relevant GDPs - methylglyoxal (MGly) or 2-furaldehyde (Fur) at the concentrations reported in traditional peritoneal dialysis fluid (Fur 0.8 μM; MGly 35 μM). AQP1 and eNOS gene expression was detected by reverse transcription-polymerase chain reaction (RT-PCR). AQP1 protein was detected by western blot. Moreover, the effect of GDPs on AQP1 gene expression was assayed in time- and dose-dependent manners. Results Fur and MGly at the concentrations reported in traditional peritoneal dialysis fluid (Fur 0.8μM; MGly 35μM) significantly up-regulated eNOS mRNA and tended to down-regulate AQP1 mRNA in cultured endothelial cells. Western blot revealed similar results. Conclusion GDPs stimulate eNOS expression in endothelial cells without up-regulating AQP1 expression. These effects may be involved in ultrafiltration failure in long run peritoneal dialysis.

Key words: Aquaporin-1, Endothelial nitric oxide synthase

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