【Abstract】Objective To observe the clinical effect of separate membrane needle-free joints applied to hemodialysis central venous catheters. Methods From January 2017 to June 2017, 36 patients with central venous catheter for maintenance hemodialysis were chosen from the Blood Purification Center of the General Hospital of Chinese PLA. They were randomly split into observation group and control group, with 18 patients in each group. The observation group used a separate membrane needle-free joint, and the control group used a conventional small cap. The side-effects of catheter-related bloodstream infection, rupture or erosion of the catheter port, bleeding and air embolism during the use of central venous catheters, and the use of a septum needle-free closed connector on blood flow during hemodialysis were noted. Results The incidence of catheter-related bloodstream infection, catheter rupture or corrosion, bleeding and air embolism were statistically significant between the two groups (P=0.043). There was no significant difference in the effect of blood flow during treatment (arterial pressure: t= -1.71, P=0.087; venous pressure t=-1.19, P=0.236). Conclusion There is no any effect on the blood flow during treatment before and after use. The use of a separate membrane needle-free connector reduces the occurrence of catheter rupture, corrosion, bleeding and air embolism. This joint can be recommended for clinical use.