中国血液净化

›› 2008, Vol. 7 ›› Issue (9): 485-487.

• 基础研究 • 上一篇    下一篇

大鼠急性甲醇中毒视神经损伤实验研究

童宗武 陈 晋 杨崇猛 高 琨 苏少明 邱 明 高秀芳 孙 阳   

  1. 云南省玉溪市人民医院肾内科（653100)
  • 收稿日期:2008-03-27 修回日期:1900-01-01 出版日期:2008-09-12 发布日期:2008-09-12

Experimental study on optic nerve injury in rats with acute methanol intoxication

TONG Zon-gwu, CHEN Jin, YANG Chong-meng, GAO Kun, SU Shao-ming, QIU Ming, GAO Xiu-fang, SUN Yang   

  1. Department of Nephrology, Yunnan Yuxi People抯 Hospital, Yuxi 653100, China
  • Received:2008-03-27 Revised:1900-01-01 Online:2008-09-12 Published:2008-09-12

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摘要： 目的 探讨大鼠急性甲醇中毒视神经损伤时间窗及程度。方法 用SD大鼠经口灌注甲醇，灌注后分不同时段处死大鼠取血测甲醇浓度、取视神经做病理检查。结果 LD50从2h开始出现较为明显的损伤，4h后程度最重。LD0损伤最重的时间段在8h后。不论是LD0、LD50在损伤程度达到最严重之后，连续观察24h、48h、72h随着血液中甲醇浓度的降低，视神经的损伤仍然明显地持续存在。结论 急性甲醇中毒对视神经早期(0.5h)就有损伤，损伤程度和剂量浓度有关，视神经的损伤最严重出现在血液中甲醇浓度高峰值的4h后，随时间推移甲醇在大鼠体内的继续弥散，致使损害迁延。

关键词: 大鼠, 急性甲醇中毒, 视神经损伤

Abstract:

Objective To study the time course and degree of optic nerve injury in rats with acute methanol intoxication. Methods Sprague-Dawley rats were fed methanol orally and sacrificed at different point of time after the gavage. Blood samples were taken for methanol concentration measurement, and optic nerves were removed for pathological examination. Results In rats taken methanol to the dose of LD50, significant optic nerve injury began after 2 hours. The injury progressed along with time and reached the peak after 4 hours. In rats taken methanol to the dose of LD0, optic nerve injury reached the peak after 8 hours. In both groups of rats, optic nerve injury persisted even though blood methanol concentration became lower after 24h, 48h and 72h of methanol administration. Conclusion Optic nerve injury occurs as early as half an hour after acute methanol intoxication. The injury degree is related to the dose and blood concentration of methanol. Optic nerve injury reaches the peak after the presence of the highest blood methanol concentration for 4 hours. Optic nerve injury persists even though blood methanol concentration goes down after the methanol administration.

Key words: Acute methanol intoxication, Optic nerve injury

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