中国血液净化

›› 2005, Vol. 4 ›› Issue (5): 261-263.

• 基础研究 • 上一篇    下一篇

肌酐分解产物对红细胞的影响及机理探讨

胡白瑛 蒋云生 刘伏友 乔玉峰 林 霞   

  1. 410011 长沙，中南大学湘雅二医院肾内科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-05-12 发布日期:2005-05-12
  • 通讯作者: 胡白瑛

  • Received:1900-01-01 Revised:1900-01-01 Online:2005-05-12 Published:2005-05-12

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摘要：

目的 探讨肌酐分解产物对红细胞损害作用及机制。方法 培养鉴定尿毒症患者的肠道细菌， 用产生肌酐水解酶的克雷伯氏菌与肌酐共同培养。取其培养液与健康红细胞孵化，观察溶血情况，并分别加葡萄糖、三磷酸腺苷（ATP）和还原型谷光甘肽（GSH），了解以上物质对溶血的纠正情况，测定红细胞内的GSH浓度。结果 肌酐代谢产物能引起红细胞溶解（P<0.05）并降低红细胞内的 GSH（ P<0.001）； GSH可纠正溶血( P<0.001)。结论 尿毒症患者肠道细菌与肌酐作用后的代谢产物能加速红细胞破坏， GSH可减轻其对红细胞的损害。

关键词: 尿毒症, 肌酐水解酶, 红细胞, GSH

Abstract:

Objective To investigate the influence and mechanism of the products of creatinine metabolites on erythrocytes. Methods Klebsiella pneumoniae were cultured and identified from feces of ureamia patients. The creatininase were isolated.The metabolite and erythrocytes were incubated, hemolysis was observed and GSH was determined in erythrocytes. Results The metabolite can bring hemolysis of erythrocytes (P < 0.05) and GSH in erythrocytes was decreased ( P < 0.001). Hemolysis of erythrocytes can corrected by GSH ( P<0.001). Conclusions The toxicity to demage erythrocytes of the metabolite of creatinine is stronger than that of creatinine. The toxicity can be relieved with GSH. 

Key words: Creatininase, Erythrocytes, GSH 

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