中国血液净化

›› 2010, Vol. 9 ›› Issue (9): 498-502.doi: 10.3969/j.issn.1671-4091.2010.09.006

• 临床研究 • 上一篇    下一篇

N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸抑制大鼠肾间质纤维化的研究

王明奡 刘睿婵 包宇实 顾云鹤 穆素红 解汝娟   

  1. 哈尔滨医科大学附属第一医院
  • 收稿日期:2010-01-07 修回日期:1900-01-01 出版日期:2010-09-12 发布日期:2010-09-12
  • 通讯作者: 解汝娟

Study on the inhibition effect of the short peptide AcSDKP on renal interstitial fibrosis in rats

WANG Ming-ao, LIU Rui-chan, BAO Yu-shi, GU Yun-he, MU Su-hong, XIE Ru-juan   

  1. 1Department of Nephrology, 2Department of Pathology, The First Clinical Hospital Affiliated to Harbin Medical University, 150001, China
  • Received:2010-01-07 Revised:1900-01-01 Online:2010-09-12 Published:2010-09-12

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摘要：


【摘要】 目的 观察N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对单侧输尿管梗阻(UUO)所致大鼠肾间质纤维化的的改善作用并探讨其机制。 方法 将雄性Wistar大鼠36只随机分为假手术组，UUO 组，处理组(AcSDKP+UUO) ,每组各12只。于造模第3、14 d各处死大鼠6只，取其梗阻侧肾脏组织行HE染色，光镜下观察肾组织病理改变,计算肾间质纤维化指数。免疫组织化学方法检测其各组肾脏组织转化生长因子 1(transforming growth factor- 1,TGF- 1)及崞交〖《鞍 ( -smooth muscle actin, -SMA)表达，RT-PCR方法检测TGF- 1及 -SMA mRNA含量。 结果 与假手术组相比,各时间点UUO组大鼠肾脏病理损害进行性加重, AcSDKP处理后可明显减轻UUO组大鼠肾间质纤维化程度(P＜0.05)；免疫组化染色及RT-PCR显示：TGF- 1、 -SMA的蛋白及mRNA表达UUO组和处理组明显多于假手术组，但处理组较UUO组明显减轻(P＜0.05)。 结论 N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)可通过抑制TGF- 1及 -SMA表达,进而抑制大鼠肾间质纤维化，减缓肾纤维化的病程进展。

关键词: N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸, 肾间质纤维化, 转化生长因子 1

Abstract: 【Abstract】Objective To investigate the effect and mechanism of the short peptide acetyl-Ser-Asp-Lys-Pro (AcSDKP) on renal interstitial fibrosis induced by unilateral ureteral obstruction in rats. Methods Thirty-six male Wistar rats were randomly divided into sham-operation group, unilateral ureteral obstruction (UUO) group, and UUO+AcSDKP treatment group. Animal model of rat renal interstitial fibrosis was established by unilateral ureteral ligation. Rats were sacrificed after the surgery for 3 or 14 days. Histological change of the obstructed kidney was observed by H-E staining. The protein expression and localization of transforming growth factor β1 (TGF-β1) and -smooth muscle actin ( -SMA) in renal tissues were detected by immunohistochemistry. The mRNA levels of TGF-β1 and -SMA were measured by reverse transcription- polymerase chain reaction. Results Compared with sham-operated group, rats in UUO group exhibited severe renal interstitial fibrosis and higher expression of TGF-β1 and -SMA (P＜0.05), while those in UUO+AcSDKP group demonstrated less renal interstitial fibrosis, and lower expression levels of TGF-β1 and -SMA (P＜0.05). Conclusions AcSDKP treatment improves renal interstitial fibrosis in rats by down-regulating TGF-β1 and -SMA expression.

Key words: Renal interstitial fibrosis, Transforming growth factor β1