中国血液净化

中国血液净化 ›› 2014, Vol. 13 ›› Issue (11): 772-774.doi: 10.3969/j.issn.1671-4091.2014.11.008

• 基础研究 • 上一篇    下一篇

1,25-二羟维生素D3对IgA肾病大鼠白介素-4的调节作用

郑喜洁1, 罗静2 ,郭珲3 ,王利华3, 李荣山4   

  1. 071000 保定，1河北大学附属医院肾内科
    030001 太原，2山西医科大学第二医院风湿科，3肾内科
    030072 太原，4山西省人民医院肾内科山西省肾脏病研究所
  • 收稿日期:2014-07-07 修回日期:2014-08-09 出版日期:2014-11-12 发布日期:2014-11-12
  • 通讯作者: 郭珲 ghty966@126.com E-mail:zhengxijiejiede@126.com
  • 基金资助:

    山西省回国留学人员科研资助项目(2013-117)；山西省国际科技合作项目(2013081056)；山西省科技攻关项目(20130313022-3)；国家自然科学基金资助项目(81102198)

Regulation effect of 1,25- dihydroxy Vitamin D3 on interleukin-4 in rat with IgA Nephropathy

  • Received:2014-07-07 Revised:2014-08-09 Online:2014-11-12 Published:2014-11-12

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摘要： 【摘要】目的探讨1,25-二羟维生素D3 对IgA 肾病大鼠白介素-4 的调节作用。方法52 只Wister大鼠剔除死亡随机分为A 组(正常对照组)、B 组(未干预组)、C 组(强的松组)、D 组(1,25(OH)¬2D3组)、E组(强的松+1,25(OH)¬2D3组)。检测大鼠24 小时尿蛋白定量、脾脏及淋巴结IL-4 含量。结果①尿蛋白定量：A 组(正常对照组)为7.03±0.99 mg/24h；B 组(未干预组)为51.49±3.04 mg/24h；C 组(强的松组)为12.15±0.75 mg/24h；D 组(1,25(OH)2D3治疗组)为23.0±2.27 mg/24h；E 组(强的松+1,25(OH)2D3治疗组)为9.99±0.79 mg/24h；经治疗后C、D、E 组24h 尿蛋白较B 组均有显著下降(P＜0.05)，其中E 组下降最显著(P＜0.05)；②脾脏IL- 4 含量：A 组(正常对照组)1.428±0.60%；B 组(未干预组)5.10±0.08%；C 组(强的松组)2.93±0.13%；D 组(1,25(OH)2D3 治疗组)4.56±0.19%；E 组(强的松+1,25(OH)2D3治疗组)1.44±0.56%；③淋巴结IL-4 含量：A 组(正常对照组)1.50±0.13%；B 组(模型组)15.4±3.11%；C组(强的松组) 2.74±0.29%；D 组(1,25(OH)2D3 治疗组)6.55±0.66%；E 组(强的松+1,25(OH)2D3治疗组)2.32±0.34%。C、D、E 组脾脏及淋巴结IL-4 含量较B 组均有显著下降(P＜0.05)，其中E 组下降最显著(P＜0.05)。结论1,25-二羟维生素D3可能下调IgA 肾病大鼠IL-4 的表达，影响Th2细胞的调节。

关键词: 1,25二羟维生素D3, IgA肾病, 白介素-4

Abstract: 【Abstract】Objective To explore the regulation effect of 1,25-dihydroxy vitamin D3 (1,25-OH2D3) on interleukin-4 (IL-4) in rat with IgA nephropathy. Method Fifty-two Wistar rats were divided into group A (normal control group), group B (no intervention group), group C (prednisone treatment group), group D (1,
25-OH2D3 treatment group), and group E (prednisone + 1,25-OH2D3 treatment group). Urine protein/24 h, IL-4 levels in spleen and lymph nodes were assayed. Result ①Urine protein/24 h was 7.03±0.99, 51.49± 3.04, 12.15±0.75, 23.0±2.27, and 9.99±0.79 mg/24 h in group A, B, C, D, and E, respectively. After the treatment, the decrease of urine protein/24 h was more in groups C, D and E than in group B (P＜0.05), and was more in group E than in groups C and D (P＜0.05). ② IL-4 level in spleen was 1.428±0.60, 5.10±0.08, 2.93± 0.13, 4.56±0.19, and 1.44±0.56% in group A, B, C, D, and E, respectively. ③ IL-4 level in lymph nodes was 1.50±0.13, 15.4±3.11, 2.74±0.29, 6.55±0.66, and 2.32±0.34% in group A, B, C, D, and E, respectively. After the treatment, the decrease of IL-4 levels in spleen and lymph nodes were more in groups C, D and E than in group B (P＜0.05), and were more in group E than in groups C and D (P＜0.05). Conclusion 1,25-OH2D3 treatment may down-regulate IL-4 expression and thus affect the regulation by Th2 lymphocytes in rats with IgA nephropathy.

Key words: 1,25- dihydroxy Vitamin D3, IgA nephropathy, Interleukin-4