中国血液净化

中国血液净化 ›› 2019, Vol. 18 ›› Issue (10): 677-680.doi: 10.3969/j.issn.1671-4091.2019.10.005

• 临床研究 • 上一篇    下一篇

Irisin 与维持性血液透析患者肌肉减少症的相关性研究

王亚美1,刘风勋1,邢利2,程艳3,张靖华4,梁献慧1,王沛1,刘章锁1   

  1. 1. 郑州大学第一附属医院血液净化中心
    2. 郑州市人民医院肾内科
    3. 郑州市第三人民医院肾内科
    4. 郑州市第七人民医院肾内科

  • 收稿日期:2019-04-01 修回日期:2019-07-23 出版日期:2019-10-12 发布日期:2019-09-27
  • 通讯作者: 王沛wangpei146@hotmail.com E-mail:wangpei146@sina.com

The correlation between irisin and sarcopenia in maintenance hemodialysis patients

  1. 1Blood Purification Center, the First Hospital Affiliated to Zhengzhou University, Zhengzhou 450052;  2Department of Nephrology, Zhengzhou Municipal Hospital, Zhengzhou 450003;  3Department of Nephrology, the Third Peoples Hospital of Zhengzhou, Zhengzhou 450001;  4Department of Nephrology, the Seventh Peoples Hospital of Zhengzhou, Zhengzhou 450006, China
  • Received:2019-04-01 Revised:2019-07-23 Online:2019-10-12 Published:2019-09-27

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摘要：

【摘要】目的探究血清Irisin 水平与维持性血液透析(maintainence hemodialysis,MHD)患者生存率的关系及Irisin 对成肌细胞自噬的影响，为Irisin 治疗肌肉减少症提供证据。方法纳入180 例MHD 患者，根据亚洲肌肉减少症工作组(asia working groups for sarcopenia, AWGS)制定的肌肉减少症的诊断共识,分为肌肉减少症组与非肌肉减少症组，用ELISA 法检测MHD 患者血清中Irisin 的浓度，分析Irisin 水平与MHD 患者生存率的关系；Western Blot 检测硫酸吲哚酚(indoxyl sulfate，IS)诱导的成肌细胞自噬相关蛋白的表达，分析Irisin 对成肌细胞自噬的影响。结果在MHD 患者中肌肉减少症组血清Irisin 水平明显低于非肌肉减少症组(t=-4.802,P＜0.001)，经过3 年的随访，低Irisin 水平组患者的死亡率高于高Irisin 水平组患者。自噬蛋白Atg-3，Atg-12 和Beclin-1 在IS 组表达增加，随着Irisin 的浓度的增加抑制了Atg-3，Atg-12 和Beclin-1 的表达。结论低Irisin 水平的MHD 患者死亡率高；Irisin 改善IS 诱导的成肌细胞自噬。

关键词: Irisin, 维持性血液透析, 肌肉减少症, 自噬

Abstract:

【Abstract】Objective The aim of this study was to determine the relationship between serum Irisin level and survival rate in maintenance hemodialysis (MHD) patients and the effect of irisin on autophagy in myoblasts. This study was expected to provide an evidence of irisin useful for the treatment of sarcopenia. Design and Method A total of 180 MHD were recruited in this study. Based on the common recognition about the diagnosis of sarcopenia from Asia Working Groups for Sarcopenia (AWGS) in 2014, patients were divided into sarcopenia group and non-sarcopenia group. Serum irisin was determined by ELISA, and the relationship between serum Irisin and survival rate of the patients was then analyzed. The expression of autophagy related genes in murine C2C12 myoblasts induced with indoxyl sulfate (IS) were assayed by western blot, and the effect of Irisin on autophagy in myoblasts was then evaluated. Results Serum irisin was significantly lower in sarcopenia group than in non-sarcopenia group (t=-4.802, P<0.001). Mortality rate was higher in patients with lower serum Irisin than in those with higher serum irisin after the follow-up study for 3 years (χ2=7.865, P=0.010). The autophage related proteins of Atg-3, Atg-12 and Beclin-1 increased significantly in IS induced C2C12 myoblasts, and these changes could be ameliorated by the elevation of Irisin concentration. Conclusions The mortality rate was higher in MHD patients with lower serum Irisin level. Irisin ameliorated the autopahge in IS induced myoblasts.

Key words: Irisin, Maintenance hemodialysis, Sarcopenia, Autophagy

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