尿毒症血清促进脐静脉内皮细胞活性氧、白介素-6的产生及左旋肉毒碱的干预作用

隋伟; 袁伟杰; 于青; 谷立杰; 徐琴君

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中国血液净化 ›› 2007, Vol. 6 ›› Issue (10) : 534-537.

论著

尿毒症血清促进脐静脉内皮细胞活性氧、白介素-6的产生及左旋肉毒碱的干预作用

隋伟袁伟杰于青谷立杰徐琴君

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摘要

目的观察体外尿毒症血清环境诱导的脐静脉内皮细胞(HUVEC)活性氧(ROS)及白介素-6(IL-6) 的产生及左旋肉毒碱 (L-CN) 对它们的影响。方法以2、7-二氢二氯荧光素染色(DCFH)和荧光分光光度计法检测ROS强度，RT-PCR和ELISA方法检测IL-6表达，观察尿毒症血清对HUVEC的影响和不同浓度L-CN(25μM, 250μM, 1 000μM, 2 500μM)的干预作用。结果尿毒症血清环境能使细胞内ROS产生及IL-6表达明显增加；而L-CN可明显抑制尿毒症血清环境中细胞内ROS产生及IL-6表达，且有浓度依赖效应。结论尿毒症血清促使血管内皮微炎症状态的建立，L-CN可抑制其组织活性氧及白介素-6的产生，减轻尿毒症患者慢性炎症反应，对血管粥样硬化病变可能有延缓作用。

Abstract

Objective To observe the effect of uremic serum on the production of reactive oxygen species (ROS) and interleukin-6 (IL-6) in human umbilical vein endothelial cells (HUVEC), and its intervention by L-carnitine (L-CN). Methods Uremic sera mixed with various concentrations of L-CN (0, 25, 250, 1000 and 2500μM) were added to the culture medium. Semi-quantitative RT-PCR and ELISA were applied to evaluate the expression of IL-6. Cells labeled with oxidation-susceptible fluorescent probe 2,7-dichorofluoresin diacetate(DCFH) and measured by spectrofluorophotometer were used for ROS assay.Results IL-6 and ROS were increased in HUVEC in the presence of uremic serum. With the addition of L-CN (250μM～2500μM) in culture medium, the production of ROS and IL-6 in HUVEC was inhibited, and the inhibition was L-CN concentration dependent. Conclusion Uremic serum induces the micro-inflammation processes of blood vessel endothelia. L-CN inhibits the production of ROS and IL-6 in HUVEC and thus the microinflammation and atherosclerosis processes in uremic patients.