中国血液净化

›› 2008, Vol. 7 ›› Issue (12): 647-650.

• 基础研究 • 上一篇    下一篇

肝细胞生长因子对腹膜透析腹膜纤维化防治作用的研究

杜烨辉 边晓慧   

  1. 辽宁省锦州市中心医院肾内科；中国医科大学附属第二医院
  • 收稿日期:2008-09-20 修回日期:1900-01-01 出版日期:2008-12-12 发布日期:2008-12-12

The effect of hepatocyte growth factor in the prevention and treatment of peritoneal fibrosis in peritoneal dialysis

DU Ye-hui, BIAN Xiao-hui   

  1. 1Department of Nephrology, Jinzhou Central Hospital, Jinzhou, Liaoning 121001, China; 2Department of Nephrology, Second Affliated Hospital of China Medical University, Shenyang, Liaoning 110000, China
  • Received:2008-09-20 Revised:1900-01-01 Online:2008-12-12 Published:2008-12-12

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摘要： 目的 探讨肝细胞生长因子对腹膜透析腹膜纤维化的防治作用及其机制。方法 ①MTT法测定肝细胞生长因子对腹膜间皮细胞增殖的影响；② ELISA 法检测细胞培养液中FN、TGF-β1及PAI一1水平； ③ RT-PCR法检测FN、PAI-1及TGF-β1 mRNA的表达。 结果 肝细胞生长因子浓度>30ng／ml时，可以改善高糖对腹膜间皮细胞增殖的抑制作用。腹透液组FN、PAI-1、TGF-β1蛋白水平显著升高，肝细胞生长因子组FN、PAI-1、TGF-β1蛋白水平均显著减少。腹透液组高糖上调腹膜间皮细胞FN、PAI-1、TGF-β1 mRNA的表达，肝细胞生长因子组使FN、PAI-1、TGF-β1 mRNA的表达水平下调。结论 肝细胞生长因子能改善高糖对腹膜间皮细胞增殖的抑制作用，同时可以使腹膜间皮细胞FN、PAI-1、TGF-β1的表达水平下调。

关键词: 肝细胞生长因子, 腹膜透析, 腹膜纤维化

Abstract: Objective To explore the effect and mechanism of hepatocyte growth factor in the prevention and treatment of peritoneal fibrosis. Method MTT method was used to measure the proliferation of cultured rat peritoneal mesothelial cells (RPMC) after stimulation by hepatocyte growth factor. ELISA was applied to examine the quantity of FN, TGF-β1 and PAI-1 in the cultured supernatants. RT-PCR was used to detect the expression of FN mRNA, PAI-1 mRNA, and TGF-β1 mRNA in RPMC. Results Hepatocyte growth factor (>30ng/ml) improved the inhibition of RPMC proliferation induced by high glucose. In RPMC incubated in dialysate containing high glucose (4.25%), FN, TGF-β1 and PAI-1 in cultured medium increased significantly, and FN, TGF-β1 and PAI-1 mRNA in these cells also increased. In RPMC incubated with hepatocyte growth factor, however, FN, TGF-β1 and PAI-1 in cultured medium decreased with the down-regulation of FN, TGF-β1 and PAI-1 mRNA in these cells. Conclusion Hepatocyte growth factor improves the inhibition of RPMC proliferation induced by high glucose, and down-regulates the expression of FN, TGF-β1 and PAI-1 in RPMC.

Key words: Peritoneum, Fibrosis

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