单皮层聚醚砜膜培养HepG2细胞的初步研究

›› 2008, Vol. 7 ›› Issue (12): 651-654.

单皮层聚醚砜膜培养HepG2细胞的初步研究

杨光辉张世昌王英杰刘涛孙树东

第三军医大学西南医院全军感染病研究所；四川大学高分子科学与工程学院

收稿日期:2008-09-12 修回日期:1900-01-01 出版日期:2008-12-12 发布日期:2008-12-12
通讯作者: 王英杰

A preliminary study of polyethersulfone membrane with a single-layer skin supporting the growth of HepG2 cells

YANG Guang-hui1, ZHANG Shi-chang1, WANG Ying-jie1, LIU Tao1, SUN Shu-dong

1 Institute of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing, 400038; 2College of Polymer Science and Engineering, Sichuan University, Chendu 610065, China

Received:2008-09-12 Revised:1900-01-01 Online:2008-12-12 Published:2008-12-12

摘要/Abstract

摘要： 目的评价单皮层聚醚砜(PES)膜对肝细胞培养的支持作用，为其用于生物人工肝支持系统生物反应器的制备奠定基础。方法通过显微镜观察制备的平板膜结构，在PES膜上培养HepG2细胞，检测培养细胞的形态、生长曲线、白蛋白合成、戊醚试卤灵转化等功能。结果制备的PES膜为单皮层非对称性多孔结构；PES膜上HepG2细胞的生长曲线典型，在8d的培养时间能增殖6至7倍；细胞在PES膜上粘附呈多细胞聚集生长，部分细胞延伸入表面微孔内；PES膜上培养细胞LDH漏出量与对照无显著差异。PES膜上培养HepG2细胞具有较强的蛋白合成和生物转化功能。结论 PES平板膜能较好的支持肝细胞的粘附、生长、增殖和功能的表达，可用于中空纤维的制备。

关键词: 聚醚砜膜, 单皮层, HepG2细胞, 中空纤维

Abstract: Objective To evaluate whether polyethersulfone (PES) membrane is capable of supporting hepatocyte growth and suitable to be used as the bioartificial liver support systems. Methods The structure of PES plate membrane was observed microscopically. HepG2 cells grew on the PES membrane, and their morphology, growth curve, albumin secretion and the transformation by pentoxyresorufin were examined. Results Prepared PES plate membrane demonstrated a single-layer skin, porous and asymmetric structure. HepG2 cells cultured on PES membrane showed a typical growth curve and proliferated to 6-7 folds after 8 days. These cells clustered together and covered the surface of the membrane, even extended into the micropores. LDH leakage had no difference between the HepG2 cells growing on the membrane and those of the control group. HepG2 cells growing on the membrane showed higher protein biosynthesis and biotransformation functions. Conclusion The PES membrane is a substrate suitable for hepatocytes growing on it. It may be useful for the construction of hollow fiber bioreactors.

Key words: Single-layer skin, HepG2 cells, Hollow fiber

中图分类号:

R318.14

杨光辉张世昌王英杰刘涛孙树东. 单皮层聚醚砜膜培养HepG2细胞的初步研究 [J]. , 2008, 7(12): 651-654.

YANG Guang-hui;ZHANG Shi-chang;WANG Ying-jie;LIU Tao;SUN Shu-dong. A preliminary study of polyethersulfone membrane with a single-layer skin supporting the growth of HepG2 cells[J]. , 2008, 7(12): 651-654.