中国血液净化

中国血液净化 ›› 2013, Vol. 12 ›› Issue (05): 271-274.

• 基础研究 • 上一篇    下一篇

血必净对高糖作用下大鼠腹膜间皮细胞增殖及细胞因子表达的影响

陈硕1,周红丽1,马健飞2   

  1. 1. 辽宁医学院附属一院
    2. 中国医科大学附属第一医院肾内科
  • 收稿日期:2013-02-16 修回日期:2013-01-16 出版日期:2013-05-12 发布日期:2013-07-01
  • 通讯作者: 陈硕 chenshuo-2009@hotmail.com E-mail:beibei520hanyu@163.com
  • 基金资助:
    辽宁医学院附属一院青年基金

Effects of Xuebijing on the proliferation, cytokine expression of Rat Peritoneal Mesothelial Cells cultured in High Glucose Concentration

  • Received:2013-02-16 Revised:2013-01-16 Online:2013-05-12 Published:2013-07-01

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摘要： 【摘要】目的 观察血必净对高糖刺激后腹膜间皮细胞(PMC)增殖、高迁移率族蛋白-1(HMGB-1)及转化生长因子-β1 (TGF-β1)表达的影响。方法 原代培养PMC；并用MTT和ELISA法测定血必净对高糖(2.5%葡萄糖)作用下PMC增殖及其分泌HMGB-1、TGF-β1的影响。结果 高糖能显著抑制PMC的增殖(P<0.05)，而2～20mg/mL的血必净能部分逆转高糖对PMC增殖的抑制作用(P<0.05)；高糖能显著增加PMC表达HMGB-1和TGF-β1 (均P<0.05)，2～20mg/mL的血必净能明显减弱高糖上调HMGB-1和TGF-β1的作用(均P<0.05）。结论 血必净可能通过逆转高糖对PMC的增殖抑制作用，抑制高糖上调PMC表达炎症介质HMGB-1和致纤维化因子TGF-β1的作用，进而修复高糖所致的PMC的损伤，延缓腹膜纤维化，为改善间皮细胞损伤和防治腹膜纤维化提供实验依据。

关键词: 血必净, 腹膜间皮细胞, 细胞增殖, HMGB-1, TGF-β1

Abstract: 【Abstract】Objective To observe the effects of Xuebijing on the inhibition of cell proliferation and the expression of high mobility group box-1 protein(HMGB-1) and transforming growth factor-β1(TGF-β1) in rat peritoneal mesothelial cells(PMCs) cultured in high glucose concentration. Methods PMCs were isolated from rat colic omentum and cultured in high glucose concentration(2.5%glucose). MTT method was processed to observe the effects of Xuebijing(2,10,20mg/mL)on the cell proliferation. ELISA analysis were performed to investigate the change of HMGB-1 and TGF--β1 concentration in the culture medium. Results High glucose concentration of 2.5% could significantly inhibit cell proliferation as well as induce the expression of HMGB-1 and TGF-β1 in PMCs（P<0.05），which could be partially reversed by Xuebijing of 2～20mg/mL（P<0.05）.Conclusion Xuebijing could reverse both the cell proliferation inhibition and cytokine like HMGB-1 and TGF-β1 induction by high glucose in PMC，and repair the cell damage induced by high glucose consequently.

Key words: Xuebijing, peritoneal mesothelial cell, proliferation, high mobility group box-1 protein, transforming growth factor-β1