中国血液净化

中国血液净化 ›› 2013, Vol. 12 ›› Issue (05): 282-286.

• 综述 • 上一篇    下一篇

维持性血液透析患者感染丙型肝炎病毒基因分型特点及意义

陈嘉,何永成,栾韶东   

  1. 深圳市第二人民医院
  • 收稿日期:2012-09-17 修回日期:2012-11-05 出版日期:2013-05-12 发布日期:2013-07-01
  • 通讯作者: 栾韶东 E-mail:luanshaodong@medmail.com.cn
  • 基金资助:
    深圳市维持性血液透析患者丙型肝炎病毒感染危险因素及传播途径的前瞻队列性研究

Characteristics of HCV Genotyping and Its Clinical Significance in Maintenance Hemodialysis Patients

  • Received:2012-09-17 Revised:2012-11-05 Online:2013-05-12 Published:2013-07-01

RichHTML

0

PDF

246

摘要： 1989 年Choo 等利用分子克隆技术获得丙型肝炎病毒（Hepatitis C Virus, HCV）的编码抗原, 并建立了血清抗-HCV 抗体检测方法，HCV已经成为非甲非乙型肝炎（non- A non- B hepatitis）的主要病原[1] 。根据HCV 基因序列差异的多少，将HCV 划分为不同的基因型和基因亚型，在世界不同地区至少存在6 个主要基因型与70多个亚型[2]。现阶段检测HCV基因型的分子生物学方法有限制性片段长度多态性、测序法及反相线性探针杂交法等。HCV核酸序列分析已用于维持性血液透析患者感染HCV的传播模式研究。HCV 基因分型不但有助于判断HCV感染及传播途径，也是干扰素抗病毒治疗的一个重要的疗效预测指标，而且与其致病性、肝硬化及肝癌发生及长期生存率密切相关。HCV分子病毒学（Molecular Virology）的研究已经证实血液透析患者之间HCV的相互传播的存在[3]。本文将就维持性血透患者感染HCV的基因分型特点及意义综述如下。

关键词: 维持性血液透析, 丙型肝炎病毒, 肝炎, 基因分型

Abstract: The epidemiology of hepatitis C virus(HCV) infection in hemodialysis patients has changed dramatically worldwide since the identification of HCV as the major pathogen of non-A non-B hepatitis in 1989. On the basis of the similarity of nucleotide sequences, HCV was classified into six genotypes and approximately seventy subtypes. These genotypes have distinct geographical distributions. The molecular biology approach to detect hepatitis C virus genotype include restriction fragment length polymorphisms, direct sequencing, reverse hybridization line probe assay and so on. HCV genotypes have unique patterns of geographic distribution and are associated with differences in response to interferon treatment . HCV genotypes are also closely associated with pathogenicity, cirrhosis, hepatocarcinogenesis and survival rate of hemodialysis patients. Nosocomial transmission of HCV among hemodialysis patients has been unequivocally demonstrated by molecular virology studies. This article will review characteristics of HCV genotyping and its clinical significance in maintenance hemodialysis patients.

Key words: Maintenance hemodialysis, HCV, Hepatitis, Genotyping