中国血液净化

›› 2007, Vol. 6 ›› Issue (9): 484-486.

• 论著 • 上一篇    下一篇

维持性血液透析患者外周血调节性T细胞的表达

眭维国 孙燕燕 黄 河 陈洁晶 蓝慧娟 戴 勇

  

  1. 541002 桂林，解放军181医院全军肾移植与血液净化中心
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-09-12 发布日期:2007-09-12

  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-12 Published:2007-09-12

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摘要： 目的 探讨维持性血液透析患者体内调节性T细胞表达水平及意义。方法 采用流式细胞术测定解放军第181医院全军肾移植与血液净化中心13例维持性血液透析患者血液透析前、血液透析后外周血CD4+T细胞占淋巴细胞的比例及CD4+CD-127Treg、CD4+CD25+CD127-Treg占CD4+T细胞的比例。结果 维持性血液透析患者血液透析前CD4+T细胞占淋巴细胞的比例高于健康对照组，CD4+CD127-Treg、CD4+CD25+CD127-Treg占CD4+T细胞的比例低于健康对照组，血液透析后CD4+T的比例下降，CD4+CD25+CD127-Treg的比例有所上升。结论 尿毒症患者外周血CD4+T数量增高，CD4+CD127-Treg、CD4+CD 25+CD127-Treg数量降低，免疫功能紊乱，而血液透析有利于改善机体的免疫功能。

关键词: 尿毒症, 血液透析, CD4+CD127-调节性T细胞, 流式细胞仪

Abstract: Objective To investigate the significance of CD4+CD127- regulatory T cells in peripheral blood of maintenance hemodialysis patients. Methods The peripheral blood samples were collected from 13 maintenance hemodialysis patients, before trans-analysis and after. The CD4+CD127-Treg and CD4+CD25+CD127-Treg ratios in CD4+T were detected by using flow cytometry. Results The CD4+T ratios in lymphocyte of maintenance hemodialysis patients were higher than healthy controls group before trans-analysis and the CD4+CD127-Treg and CD4+CD25+CD127-Treg ratios in CD4+T were lower, but CD4+T ratios decreased and CD4+CD25+CD127-Treg ratios increased after trans-analysis. Conclusion The number of CD4+T is increasing, CD4+CD127-Treg is decreasing in maintenance hemodialysis patients and their immune is disturbing, and hemodialysis can improve immune function of the patients.

Key words: CD4+CD127-regulatory T cells, Flow cytometer

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