多种半透膜材料肝细胞相容性的对比研究

›› 2009, Vol. 8 ›› Issue (1): 38-42.

多种半透膜材料肝细胞相容性的对比研究

杨光辉张世昌王英杰刘涛孙树东

第三军医大学西南医院全军感染病研究所；四川大学高分子科学与工程学院

收稿日期:2008-09-08 修回日期:1900-01-01 出版日期:2009-01-12 发布日期:2009-01-12

A comparison study of hepatic-compatibility among several semi-permeable membranes

YANG Guang-hui, ZHANG Shi-chang, WANG Ying-jie, LIU Tao, SUN Shu-dong

1Institute of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing, 400038; 2College of Polymer Science and Engineering, Sichuan University, Chendu, 610065

Received:2008-09-08 Revised:1900-01-01 Online:2009-01-12 Published:2009-01-12

摘要/Abstract

摘要： 【摘要】目的筛选出与肝细胞有较好生物相容性的半透膜材料，以用于生物人工肝支持系统中空纤维型反应器的制备。方法选择聚醚砜(PES)、聚砜(PSF)、醋酸纤维素(CA)、聚偏二氟乙烯(PVDF)、聚丙烯(PP)、混合纤维素酯(CNA)等有代表性的6种半透膜材料进行肝细胞(L02细胞)培养，同时以聚苯乙烯培养板作为对照组，检测细胞LDH漏出量和DNA Ladder细胞凋亡评价膜材料的细胞毒性，观察各种膜材料上L02细胞的贴壁率、细胞活性、DNA总量等。结果各种膜材料未见明显细胞毒性；L02细胞在PES膜上的贴壁率显著高于CA、PVDF、PP、CNA膜(P＜0.05)，与对照组和PSF膜无显著差异；PES膜上贴壁细胞数明显较CA和CNA膜多；PES和PSF膜上细胞DNA总量显著高于CA、PP、CNA膜(P＜0.05)；在7天的培养时间内，PES膜上和对照组的细胞活性最高，显著高于CA、PVDF、PP、CNA膜(P＜0.05)。结论在6种半透膜材料中，PES是肝细胞相容性最佳的半透膜材料，适宜用于生物反应器中空纤维的制备。

关键词: 半透膜, 肝细胞, 相容性, 中空纤维

Abstract: 【Abstract】 Objective To screen out a semi-permeable membrane with the best hepatic-compatibility suitable for making hollow fiber bioreactor in bioartificial liver supporting system. Methods Human liver cell lines (L02) were cultured on 6 different semi-permeable membranes made of polyethersulfone (PES), polysulfone (PSF), cellulose acetate (CA), polyvinylidene fluoride (PVDF), polypropylene (PP) and cellulose nitrate acetate (CNA). The cells cultured on polystyrene plate were used as a control group. Cytotoxicity of the membrane was evaluated by the data of lactate dehydrogenase (LDH) leakage and DNA ladder. Adherence ratio, cell viability, protein synthesis and DNA amount of the L02 cells were determined. Results No cytotoxicity was found to the L02 cells growing on the 6 membranes. The adherence ratio of L02 cells cultured on PES membrane was significantly higher than that of the cells cultured on CA, PVDF, PP and CAN membranes, and was similar to that of the control group and the cells cultured on PSF membrane. The amount of total DNA from L02 cells growing on PES and PSF membranes was higher than that from the cells growing on CA, PP and CNA membranes. On the 7th day of culture, the cell viability was higher in cells growing on PES membrane than those on CA, PVDF, PP and CAN membranes. Conclusion PES membrane showed the best hepatic-compatibility among the 6 membranes tested. Therefore, PES is suitable for making hollow fiber bioreactor in bioartificial liver supporting system.

Key words: Hepatocytes, Compatibility, Hollow fiber

杨光辉张世昌王英杰刘涛孙树东. 多种半透膜材料肝细胞相容性的对比研究[J]. , 2009, 8(1): 38-42.

YANG Guang-hui;ZHANG Shi-chang;WANG Ying-jie;LIU Tao;SUN Shu-dong. A comparison study of hepatic-compatibility among several semi-permeable membranes[J]. , 2009, 8(1): 38-42.