中国血液净化

中国血液净化 ›› 2013, Vol. 12 ›› Issue (05): 251-253.doi: 10.3969/j.issn.1671-4091.2013.05.00

• 临床研究 • 上一篇    下一篇

三醋酸纤维素膜与聚砜膜低通量透析器对细菌DNA片段的通透性研究

陈欢1,赵班1,燕宇2,王梅2   

  1. 1. 卫生部北京医院肾内科
    2. 北京大学人民医院
  • 收稿日期:2013-03-04 修回日期:2013-03-13 出版日期:2013-05-12 发布日期:2013-07-01
  • 通讯作者: 王梅 wangmeil949@163.com E-mail:wangmei1949@163.com

The in vitro study on bacteria DNA transfer across dialyzers of different dialysis membrane

  • Received:2013-03-04 Revised:2013-03-13 Online:2013-05-12 Published:2013-07-01

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摘要： 目的　通过体外实验观察三醋酸纤维素膜和聚砜膜低通量透析器对细菌DNA片段的通透性，比较两种透析器对细菌DNA片段的截留能力。　方法　以透析器类型分为三醋酸纤维素膜/低通量(130G)和聚砜膜/低通量(F6hps)两组。体外模拟血液透析4小时，血液侧为无菌生理盐水500ml,流速200ml/min。透析液侧为无菌生理盐水1000ml。以大肠埃希氏菌分别为2.06×102，1.603×103，2.671×103，1.336×104cells/ml浓度的混悬液作为污染透析液，透析液流速200ml/min。采用鲎试剂动态比浊法检测透析液和血液侧样本内的内毒素浓度，以超微量分光光度计检测血液侧样本的细菌DNA浓度，并对两组透析器血液侧的细菌DNA浓度进行比较。　结果　①透析4小时后，两组透析器的血液侧透后样本的内毒素检测均为阴性。但细菌DNA浓度升高，并随透析液中污染菌浓度增加呈现上升趋势。②在透析液细菌浓度在1.603×103，2.671×103，1.336×104cells/ml浓度时，130G组血液侧样本的细菌DNA浓度明显高于F6hps组，两组间比较，t值分别为7.982,4.264,10.499,P值分别为0.001,0.013,<0.001,差异显著性有统计学意义。　结论　①细菌DNA比内毒素对三醋酸纤维膜和聚砜膜透析器的穿透能力更强。②与聚砜膜透析器相比，三醋酸纤维素膜对细菌DNA有更高的通透性。

关键词: 细菌DNA, 三醋酸纤维膜, 聚砜膜, 内毒素

Abstract: 【Abstract】Objective To assess the transfer of bacteria DNA across cellulose triacetate(CTA, low-flux) and polysulfone(low-flux) membranes. Methods Two groups based on the dialyzer of different membrane were used in this study. Experiments were carried out in saline-saline model. The dialysate was contaminated with E.Coli at 4 levels(2.06×102，1.603×103，2.671×103，1.336×104cells/ml), in vitro dialysis was performed under diffusive condition for 4 hours. ultraviolet spectrophotometry and LAL test were used respectively to detect bacteria DNA and endotoxin level in blood side samples. Comparing the difference of bacteria DNA levels in blood side samples between two groups. Results Dialysate challenged with E.Coli at 4 levels, Increased bacteria DNA concentration were observed in all blood side samples, whereas LAL negative after 4-hour dialysis. Bacteria DNA concentration in blood side samples increased along with the elevated extent of E.Coli in dialysate. E.Coli level in dialysate from 103～104cells/ml, higher bacteria DNA concentrations were observed in 130G group than in F6hps group. The difference is significant between them(P<0.05). Conclusion Under identical conditions for two dialyzers, bacteria DNA have better permeabitlity across membrane than endotoxin. CTA low-flux dialyzer has more permeability of bacteria DNA than Polysulfone low-flux dialyzer.

Key words: bacteria DNA, cellulose triacetate membrane, polysulfone membranes, endotoxin